The Complement Component 4 Binding Protein α Gene: A Versatile Immune Gene That Influences Lipid Metabolism in Bovine Mammary Epithelial Cell Lines.

Complement component 4 binding protein α () is an immune gene which is responsible for the complement regulation function of by binding and inactivating the Complement component C4b () component of the classical Complement 3 () invertase pathway. Our previous findings revealed that was differentially expressed by comparing the transcriptome in high-fat and low-fat bovine mammary epithelial cell lines (BMECs) from Chinese Holstein dairy cows. In this study, a gene knockout BMECs line model was constructed via using a CRISPR/Cas9 system to investigate the function of in lipid metabolism.

Syndecan-3 Coregulates Milk Fat Metabolism and Inflammatory Reactions in Bovine Mammary Epithelial Cells through AMPK/SIRT1 Signaling Pathway.

Transcriptome sequencing showed that syndecan-3 () was differentially expressed in high-fat and low-fat mammary epithelial cells of Chinese Holstein cows. Previous studies found that plays an important role in inflammatory diseases and virus infection. However, those studies did not confirm whether or not the functional gene , which plays an important role in regulating milk fat metabolism, has an effect on susceptibility to breast tissue diseases.