Screening ssDNA Aptamers Against Human Vascular Endothelial Factor 165 via Semirational Design.

As a valid tumor marker, vascular endothelial growth factor 165 (VEGF165) is an effective therapeutic target for anticancer treatments. Aptamers hold great promise for the development of anti-VEGF strategies. In this study, anti-VEGF165 ssDNA aptamers were screened using a semirational design and a multilevel screening strategy.

[Production of high-purity recombinant human vascular endothelial growth factor (rhVEGF165) by Pichia pastoris].

Vascular endothelial growth factor (VEGF165) is a highly specific vascular endothelial growth factor that can be used to treat many cardiovascular diseases. The development of anti-tumor drugs and disease detection reagents requires highly pure VEGF165 (at least 95% purity). To date, the methods for heterologous expression and purification of VEGF165 require multiple purification steps, but the product purity remains to be low.

"Yiqi Huayu, Wenyang Lishui" Prescription (YHWLP) Improves the Symptoms of Chronic Obstructive Pulmonary Disease-Induced Chronic Pulmonary Heart Disease by Inhibiting the RhoA/ROCK Signaling Pathway.

Background: Chronic pulmonary heart disease (CPHD) is a common type of heart disease. In China, chronic obstructive pulmonary disease (COPD) is one of the main causes of CPHD. At present, there is no specific therapy for COPD-induced CPHD, so it is of great importance to identify a new therapy for CPHD.

The tongue features associated with chronic kidney disease.

Background: Traditional Chinese medicine (TCM) tongue diagnosis plays an important role in differentiation of symptoms because the tongue reflects the physiological and pathological condition of the body. The automatic tongue diagnosis system (ATDS), which noninvasively captures tongue images, can provide objective and reliable diagnostic information. Chronic kidney disease (CKD) currently is an important global public health problem and contributor to morbidity and mortality from non-communicable diseases.

Increased Plasma Level of miR-210 as a Potential Diagnostic Marker for Chronic Obstructive Pulmonary Disease Induced Pulmonary Hypertension.

Background: Pulmonary hypertension (PH) is a commonly observed complication of chronic obstructive pulmo-nary disease (COPD) which may lead to poor prognosis of the disease. The present study aims to explore the roles of circulating miR-210 for the early diagnosis of COPD included PH.

Methods: We included 80 patients with COPD including PH, 80 patients with COPD but without PH, and 80 healthy subjects as the study population.