Development of a new sampling medium for bioaerosols.

Objective: To develop a new sampling medium for detecting of bioaerosols.

Methods: The sampling media were tested by using Escherichia coli, Staphylococcus aureus and Serratia marcescens under static and active conditions, preliminary applications were performed using AGI-10 and high volume sampler.

Results: The average recovery rates were raised to 24.

[p16INK4a expression mediated by recombinant adenovirus can induce senescence of A549 cells].

Objective: To construct E1-deletion and replication-defective human type 5 recombinant adenovirus vector and to study the effect of p16INK4a on proliferation and aging of A549 cells.

Methods: p16INK4a cDNA was cloned into pAdCMV to construct recombinant pAdCMV p16INK4a, which was co-transfected into 293 cell together with pJM17. The recombinant p16INK4a adenovirus (Ad-p16INK4a) was generated by homologous recombination and identified with duplex PCR.