[Identification and the Related Molecular Mechanism of Allele].

Objective: To investigate the identification and molecular biological mechanism of a case of allele.

Methods: The ABO blood groups of the proband and his nine family members were analyzed serologically and DNA sequencing was used to accurately determine the genotypes of these ten specimens. The cartoon models of local active center of enzymes of the GTA,GTB and the GTB mutant were constructed to explore the possible molecular mechanism leading to abnormal enzyme-catalyzed A antigen synthesis.

[Serological Characteristics and Molecular Biological Mechanism of AEL.02 Subtype].

Objective: To explore the serological characteristics and molecular biological mechanism of an a subtype specimen.

Methods: The ABO blood typing was identified by routine blood group serological and absorption/elution methods; PCR-SBT method for ABO genotyping: 7 exons of ABO gene were amplified by PCR, the amplified products were purified, and then sequencing primers were designed and the amplified products were sequenced directly for analysis; 3D molecular model was constructed and the difference of free energy (ΔΔG) was used to predict the GTA mutant stability.

Results: A antigen was not detected on erythrocytes through absorption and elution tests, which was not consistent with the serological characteristics of a, and the serological typing results were ambiguous.

Association of HLA alleles (A, B, DRB1) and HIV-1 infection in the Han population of Hubei, China.

The HIV susceptibility and resistance alleles in the HLA genes were determined by investigating the distribution characteristics of the HLA alleles (A, B, and DRB1) in HIV-infected individuals of the Han population in Hubei, and by comparing these alleles with HIV-negative individuals from the same area. A cohort of 424 HIV-1 infected individuals were chosen as study subjects, and 836 HIV-negative healthy subjects from the same area served as the control population. HLA-A, B, and DRB1 allele typing was performed using polymerase chain reaction-sequence-specific oligonucleotide probes (PCR-SSOP) and polymerase chain reaction-sequencing based typing (PCR-SBT) techniques.