Rapid screening and characterization of triterpene saponins in Acanthopanax senticosus leaves via untargeted MS and SWATH techniques on a quadrupole time of flight mass spectrometry.

This paper focused on untargeted MS, also called MS, and sequential window acquisition of all theoretical fragment-ion spectra (SWATH) fragmentations for comprehensive structural characterization of triterpene saponins (TSs) in leaves of Acanthopanax senticosus through ultra-high performance liquid chromatography electrospray quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF). [M+H], [M + NH] and [M + Na] precursor ions and the corresponding fragment ions were collected simultaneously in energy-resolved MS. SWATH fragmentation was applied as a comparable and complementary method for resolving co-eluting species.

A generic strategy based on gas phase decomposition of protonated and ammoninted precursors producing predictable MRM-MS ion pairs and collision energies for direct analysis of plant triterpene glycosides.

Optimization of multiple reaction monitoring mass spectrometry (MRM-MS) parameters of triterpene glycosides (TGs) using traditional infusion methods remains to be labor-intensive. However, it was found that mild gas phase decompositions of protonated and ammoninted precursors (DPAP) of TGs could produce a series of abundant dehydrated product ions of aglycones ([A+H-nHO] (n = 0, 1, 2, 3…)) with high efficiency and stability. Based on these considerations and findings, an innovative ESI-MRM-DPAP-MS strategy was devised on a QTRAP 4000 instrument allowing for rapid the qualitative and quantitative analysis of plant TGs.

Simultaneous Separation of Eight Lignans in Forsythia suspensa by β-Cyclodextrin-Modified Capillary Zone Electrophoresis.

The aim of the study was to develop an alternative capillary zone electrophoresis (CZE) for simultaneous determination of phillyrin (), phillygenin (), epipinoresinol-4--β-glucoside (), pinoresinol-4--β-glucoside (), lariciresinol (), pinoresinol (), isolariciresinol () and vladinol D () in . The structural types of lignans - could be attributed to bisepoxylignans (- and ), monoepoxylignans ( and ) and cyclolignan (). The major difficulties in the CZE separation of - could be summarization as the simultaneous presence of free lignans (, and -) and lignan glucosides ( and ) and simultaneous occurrence of two pairs of isomers ( and as well as and ).

Early pregnancy following multidrug regimen chemotherapy in a gestational trophoblastic neoplasia patient: A case report.

Rationale: Gestational trophoblastic neoplasia is a group of rare tumors that can be cured using chemotherapy. The use of artificial contraception for at least 1 year is recommended not only due to the high recurrence rate in the first year after treatment, but also because of the unclear genetic toxic effects of multidrug regimen chemotherapy on reproductive cells. There is no consensus about the contraception duration, but most patients want to have children.