Discovery of two new long chain fatty acid esters of flavonol glycoside from Pollen Typhae Carbonisatus and their protective effects on HBMECs.

ABATRACTTwo new compounds, namely, isorhamnenin-3--neohesperidin-6''-linoleic acid ester () and isorhamnenin-3--neohesperidin-6''-palmitate (), along with 17 known compounds, were isolated from Pollen Typhae Carbonisatus (PTC). Their structures were elucidated on the basis of one-dimensional (1D)-, two-dimensional (2D)-nuclear magnetic resonance (NMR), and high-resolution electrospray ionization mass spectrometry (HRESIMS) spectroscopic data analyses. The two new compounds ( and ) exhibited a protective effect in a dose-dependent manner and promoted tube generation in the oxygen-glucose deprivation/reoxygenation (OGD/R)-induced human brain microvascular endothelial cells (HBMECs).

Isosativene and sativene sesquiterpene derivatives from Dendrobium nobile.

Two unusual isosativene sesquiterpene derivatives, named dendronobilol A (1) and dendronobilside A (2), and two unusual sativene sesquiterpene derivatives, named dendronobilsides B (3) and C (4), had been isolated from the stems of Dendrobium nobile. The structures of all the compounds were established using spectroscopic methods and by comparison with literature data, and their absolute configurations were confirmed via single-crystal X-ray diffraction data and electronic circular dichroism (ECD) calculations. Dendronobilol A (1) and dendronobilside A (2) possessed a unique tricyclo[4.

Two new sesquiterpene glycosides from the stems of and their anti-inflammatory activity.

Two new sesquiterpene glycosides, 8,12,15-trihydroxycopacamphan-15---D-glucopyranoside () and dendrobiumane C-11---D-glucopyranoside (), along with three known terpenoids () were isolated from the aerial stems of . Their structures were elucidated based on NMR-spectroscopic and HR-MS analyses. All compounds could reduce the levels of NO, TNF- and IL-1 in LPS-induced RAW264.

Two new anti-inflammatory trachylobane diterpenoids from .

Two new rare trachylobane euphoratones A-B (-), together with five known diterpenoids (compounds ), were isolated from the aerial parts of . Their structures were unambiguously elucidated through HRESIMS, 1D and 2D NMR spectral analysis. Compounds , , and showed weak anti-inflammatory activities (IC 77.

Two new catechins from Zijuan green tea enhance the fitness and lifespan of insulin-like signaling pathways.

Green tea polyphenols show positive effects on human health and longevity. However, knowledge of the antiaging properties of green tea is limited to the major catechin epigallocatechin gallate (EGCG). The search for new ingredients in tea with strong antiaging activity deserves further study.

Phytochemical and pharmacological review of diterpenoids from the genus Euphorbia Linn (2012-2021).

Ethnopharmacological Relevance: Euphorbia is one of the major genera in angiosperms, which is widely distributed all over the world, including Asia, Africa and Central and South America. The roots or tubers of Euphorbia are famous for medicinal purposes, especially in China. Many of them, such as Euphorbia pekinensis Rupr, Euphorbia fischeriana Steud and Euphorbia Kansui S.

Physicochemical, morpho-structural, and biological characterization of polysaccharides from three spp.

species, including , , and , are edible plants with medicinal purposes, which have long been consumed as food due to their high nutritional value. In this study, polysaccharides from (PCP), (PKP) and (PSP) were obtained, and their physicochemical properties and biological activities were investigated. Our results demonstrated that PCP, PKP, and PSP consist of major fructose and minor glucose, galacturonic acid, and galactose in different molar ratios with the molecular weights of 8.

Ten picrotoxane-type sesquiterpenoids from the stems of Dendrobium wardianum Warner.

Ten undescribed picrotoxane-type sesquiterpenoids, dendrowardins A-J, together with two known ones, were isolated from the stems of Dendrobium wardianum Warner (Orchidaceae). Dendrowardins A-D feature the unusual 5,2-δ-lactone and additionally dendrowardins C-D are the first examples containing the 11,10-γ-lactone moiety. The structures were established using spectroscopic methods and by comparison with literature data.

[Chemical constituents from ethyl acetate extraction of Prunus mume].

Eight compounds were isolated from the ethyl acetate extraction of Prunus mume by column chromatography. On the basis of physicochemical properties and spectrum analysis, these compounds were identified as isoquercitrin-6″-O-benzoate(1), pinoresinol(2), naringin(3), ethyl-β-D-glucopyranoside(4), astragalin(5), quercetin(6), hypericin(7), and rutin(8). Among them, compound 1 was a new natural product, and compounds 2-5 were isolated from this plant for the first time.

Two new acetoisovanillone glycosides from the water-soluble fraction of .

The cortex root of , is used as a traditional Chinese medicine for treating female diseases. Phytochemical investigation of the water-soluble fraction of the plant led to the isolation of two new acetoisovanillone glycosides: acetoisovanillone-3D-glucopyranoside () and 2-hydroxy-acetoisovanillone-3---D-glucopyranoside (). Their structures were elucidated by extensive spectroscopic methods.

Chemical constituents from plant endophytic fungus .

Five new natural compounds (-) along with four known ones, involving dibenzo--pyrone derivatives, a benzo--pyrone derivative and an amide-type compound were obtained from , an endophyte isolated from The structures of these isolates were elucidated by intensive analysis of spectroscopic data including NMR, HRMS (ESI and EI), UV and IR spectra. Compounds (-) were evaluated for their cytotoxicities against five selected human tumourtumour cell lines (A-549, MDA-MB-231, MCF-7, KB and KB-VIN), and compound exhibited activities against MDA-MB-231and MCF-7 with IC values of 20.1 M and 32.

Design, Synthesis, and Biological Evaluation of Novel Biotinylated Podophyllotoxin Derivatives as Potential Antitumor Agents.

Podophyllotoxin has long been used as an active substance for cytotoxic activity. Fourteen novel biotinylated podophyllotoxin derivatives were designed, synthesized, and evaluated for cytotoxic activity for this study. The synthesized compounds were evaluated for cytotoxic activity in the following human cancer cell lines, SW480, MCF-7, A-549, SMMC-7721, and HL-60 by MTT assay.

[A new benzophenone isolated from fibrous roots of Anemarrhena asphodeloides].

Five compounds were isolated from the fibrous roots of Anemarrhena asphodeloides by silica gel, Sephadex LH-20 and semi-HPLC column chromatography. On the basis of physic-chemical properties and spectroscopic data analysis, these compounds were identified as methyl 2-[2,4-dihydroxy-3-(4-hydroxybenzoyl)-6-methoxyphenyl]acetate(1), 4-[formyl-5-(methoxymethyl)-1H-pyrrol-1-yl]butanoate(2), perlolyrine(3),syringaresinol-4'-O-β-D-glucoside(4) and 4',6-dihydroxy-4-methoxybenzophenone-2-O-(2″),3-C-(1″)-1″-desoxy-α-L-fructofuranoside(5). Among them, 1 was a new benzophenone.

One new benzophenone and one new 1,3-diphenylpropane from the fibrous roots of Bge. and their cytotoxicity.

One new benzophenone and one new 1,3-diphenylpropane were obtained from the fibrous roots of Bge. Their structures were determined by comprehensive 1D, 2D NMR and HRESIMS data. By comparing the calculated ECD curves and OR with the experimental data the absolute configurations were determined.

Aspergilolide, a steroid lactone produced by an endophytic fungus . MBL1612 isolated from .

A new steroid lactone aspergilolide (), and nine known compounds helvolic acid (), verruculogen (), tryprostatin B (), 13-oxofumitremorgin B (), fumitremorgin C (), demethoxy fumitremorgin C (), terezine D (), aszonalenin (), 12, 13-dihydroxy-fumitremorgin C () from cultures of the endophytic fungus . MBL1612. Their chemical structures were determined by a series of extensive spectroscopic methods.

Synthesis and antitumor activity of biotinylated camptothecin derivatives as potent cytotoxic agents.

A series of biotinylated camptothecin derivatives were designed and synthesized. The key to the synthesis was achieved by employing an esterification reaction and click chemistry. All of the new derivatives were tested for cytotoxicity against five human tumor cell lines, including HL-60, SMMC-7721, A-549, MCF-7, and SW480 with IC values ranging from 0.

Mimengosides J and K: two new neuroprotective triterpenoids from the fruits of Buddleja lindleyana.

Two new 11-methoxyl substituted triterpenoids, named as mimengosides J (1) and K (2), along with seven known compounds, were isolated from the fruits of Buddleja lindleyana. Their structures were elucidated on the basis of spectroscopic analysis. In addition, the new ones were evaluated for protective effects against damage of SH-SY5Y cells induced by 1-methyl-4-phenylpyridinium ion (MPP) and the results indicated that those may be one of the candidate compositions of Buddleja lindleyana for the treatment of neurodegenerative disease.

Aspergilates A to E, second metabolites from Aspergillus sp. isolated from Paeonia ostii.

Five novel globoscinic acid derivatives, aspergilates A-E (1-5) have been isolated from endophytic fungus Aspergillus sp. derived from Paeonia ostii. The structures of aspergilates A-E were determined by in-depth analyses of NMR spectra, HRESIMS and CD experiments.

Synthesis and anticancer activity of dimeric podophyllotoxin derivatives.

Background: Podophyllotoxin is a potent cytotoxic agent and serves as a useful lead compound for the development of antitumor drugs. Several podophyllotoxin-derived antitumor agents, including etoposide, are currently in clinical use; however, their therapeutic efficacy is often limited due to side effects and the development of resistance by cancer cells. Previous studies have shown that 4β-1,2,3-triazole derivatives of podophyllotoxin exhibit more potent anticancer activity and better binding to topoisomerase-II than etoposide.

Alkaloids from flowers of Erythrina corallodendron.

Two new Erythrina alkaloids, 10-oxo-erythrinine (1) erythrinine N-oxide (2) together with 23 known ones were obtained from the flowers of Erythrina corallodendron. The structures were determined based on analysis of their spectroscopic data. All compounds were first isolated from plants of Erythrina corallodendron.

Four new glycosides from the stems of Dendrobium fimbriatum Hook.

Four new glucosides, named as gigantol-5-O-β-d-glucopyranoside (1), 9,10-dihydro-aphyllone A-5-O-β-d-glucopyranoside (2), ficusal-4-O-β-d-glucopyranoside (3), botrydiol-15-O-β-d-glucopyranoside (4), together with eight known compounds (5-12) were isolated from the n-BuOH extract of the stems of Dendrobium fimbriatum Hook. Their structures were elucidated by the analyses of spectroscopic data.

Protective effects of cycloartane triterpenoides from Passiflora edulis Sims against glutamate-induced neurotoxicity in PC12 cell.

Four new cycloartane triterpenoids, 1α,3β-dihydroxy-16-keto-24(31)-en-cycloartane (1), 31-methoxyl-passifloic acid (2), cyclopassifloside XIV (3), and cyclopassifloside XV (4), together with six known compounds (5-10) were isolated from Passiflora edulis Sims. Their structures were elucidated on the basis of extensive spectroscopic analysis. All the compounds were evaluated for protective effects against damage of PC12 cell induced by glutamate according to traditional usage of the herbal medicine, and the results indicated that cycloartane triterpenoids maybe one of the active compositions of P.

[Study on Flavonoids in Buddleja lindleyana Fruits].

Objective: To study the flavonoids in the fruits of Buddleja lindleyana.

Methods: The compounds were separated by repeated silica gel, RP-18 and Sephadex LH-20. Their structures were elucidated on the basis of chemical evidence and spectral data.

Five new phenolic compounds from Dendrobium aphyllum.

One new phenanthrene, aphyllone A (1) and four new bibenzyl derivatives, aphyllone B (2) and aphyllals C-D (3-5), together with nine known compounds (6-14), were isolated from the stems of Dendrobium aphyllum (Roxb.) C. E.