Effect of environmental hyperthermia on gastrin, somatostatin and motilin in rat ulcerated antral mucosa.

Aim: To study the effect of environmental hyperthermia on gastrin, somatostatin and motilin in rat ulcerated antral mucosa.

Methods: Forty-two Wistar rats were equally divided into six groups, according to the room temperature (high and normal) and the treatment (acetic acid, normal saline and no treatment). Levels of gastrin, somatostatin and motilin in rat ulcerated antral mucosa were measured with a radioimmunoassay method.

G and D cells in rat antral mucosa: an immunoelectron microscopic study.

Aim: To investigate the gastrin secreting cells (G cells) and the somatostatin secreting cells (D cells) of antral mucosa in rats at the ultrastructural level.

Methods: Revised immunoelectron microscopic technique was used to detect the G cells and D cells in rat antral mucosa through gastrin and somatostatin antibodies labeled by colloidal gold. Also the relevant quantitative analysis regarding the granular number of colloidal gold in G cells and in D cells was conducted.

[Construction and application of colorectal polyp database].

Objective: To study the construction and application of computerized database of colorectal polyp in the clinical management and research of this disease.

Method: A colorectal polyp database and its management system was constructed on the basis of Microsoft Access 2000. Clinical, endoscopic and pathological data, which went through standardized and elemental processing, of 2 627 cases (4 850 records) of colorectal polyp collected from 1990 to 2000 in Nanfang Hospital was entered into this database.

Effect of high temperature on gastrin, somatostatin and motilin production in ulcerous gastric antral mucosa of rats.

Objective: To investigate the changes of gastrin, somatostatin and motilin production in the gastric antral mucosa of rats with experimental gastric ulcer.

Methods: Rat models of gastric ulcer model were induced successfully by injection of acetic acid into the gastric antral wall of 2 groups of Wistar rats (7 in each group) that were subjected to environment of either high or normal temperature. Another 2 groups of rats (n=7) receiving normal saline injection in the same manner, along with still another 2 groups (n=7) without any treatment, all of which were kept under conditions with different temperatures accordingly, constituted the control groups.

Gastrin, somatostatin, G and D cells of gastric ulcer in rats.

Aim: To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats.

Methods: Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model was induced by acetic acid successfully.