Liver injury in COVID-19: Holds ferritinophagy-mediated ferroptosis accountable.

Even in patients without a history of liver disease, liver injury caused by coronavirus disease 2019 (COVID-19) is gradually becoming more common. However, the precise pathophysiological mechanisms behind COVID-19's liver pathogenicity are still not fully understood. We hypothesize that inflammation may become worse by cytokine storms caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection.

[Changes of brain CT imaging of cerebral cysticercosis patients during the period of anti-cysticercus chemotherapy].

Objective: To investigate the changes of brain CT imaging in patients with cerebral cysticercosis during the period of anti-cysticercus chemotherapy.

Methods: A total of 380 inpatients that were conformed to the practical diagnostic criteria of cerebral cysticercosis were enrolled in this study in the Third Affiliated Hospital of Shandong Academy of Medical Sciences from May 2010 to May 2015. All the patients were given anti-cysticercus chemotherapy (albendazole and praziquantel).

[Allele genetypes and homology analysis of MSP-1 and CSP gene of in Shandong Province].

Objective: To analyze the genotypes and homology of MSP-1 and CSP gene of in Shandong Province, so as to provide the evidence for case traceability.

Methods: A total of 12 blood samples were collected from -infected cases in Shandong Province in 2011. Parasite genomic DNA was extracted.

Liberal versus restrictive fluid management in abdominal surgery: a meta-analysis.

Purpose: This study compared perioperative restrictive fluid therapy to liberal (conventional) fluid therapy in patients undergoing major abdominal surgery and investigated the rate of post-operative morbidity (complication rates), recovery (time to flatus), and the length of hospital stay.

Methods: The Medline, PubMed, Cochrane, and EMBASE databases were searched until June 18, 2015. Randomized controlled trials, two-arm prospective studies, and retrospective studies were included in our analyses.

[Extraction Method of Malaria Parasite DNA from Preserved Positive Blood Smears].

Objective: To develop a method for DNA extraction from malaria parasites on preserved blood smears, to provide basis for research on malaria genetic traceability.

Methods: The improved DNA extraction kit (QIAamp DNA Mini Kit) was used to extract plasmodium DNA from 41 giemsa-stained blood smears, and the extraction was compared with that using the Chelex-100 and Na(2)HPO(4) methods. Nested PCR was used to amplify small subunit ribosomal RNA to identify Plasmodium parasite.

Establishment of lymphatic filarial specific IgG4 indirect ELISA detection method.

Objective: To establish the lymphatic filarial specific IgG4 indirect ELISA detection method and develop the kits.

Methods: ELISA and the developed specific IgG4 reagent was used to explore the best way for detecting filarial specific IgG4. Combined with the production process of commercialized enzyme immunoassay kit to develop economical lymphatic filarial specific IgG4 test kit, and to explore the value of the kit in the laboratory.

Construction and identification of Complex DNA vaccine of hepatitis B and Toxoplasma gondii.

Objective: To construct and identify multi-gene recombinant expression vector pcDNA3-HBsAg-p30-ROP2.

Method: Primers were designed according to the gene sequences of restriction enzyme cutting site of recombinant pcDNA3-p30-ROP2 and hepatitis B surface antigen (HBsAg). The target fragment of HBsAg was amplified and cloned to expression vector pcDNA3-p30-ROP2 by restriction enzyme digestion and ligation.

[Cloning, expression, purification and identification of Toxoplasma gondii SAG2 gene in Escherichia coli].

Objective: To construct a recombinant plasmid containing surface antigen 2(SAG2) gene of Toxoplasma gondii and express it in Escherichia coli.

Methods: The truncated SAG2 gene was amplified from the genomic DNA of T. gondii RH strain and cloned into plasmid pGEX-4T.

Protection of MES23.5 dopaminergic cells by obestatin is mediated by proliferative rather than anti-apoptotic action.

Obestatin is an endogenous peptide sharing a precursor with ghrelin. This study aims to investigate whether and how obestatin protects MES23.5 dopaminergic cells against 1-methyl-4-phenylpyridinium (MPP(+))-induced neurotoxicity.

Methods of patient warming during abdominal surgery.

Background: Keeping abdominal surgery patients warm is common and warming methods are needed in power outages during natural disasters. We aimed to evaluate the efficacy of low-cost, low-power warming methods for maintaining normothermia in abdominal surgery patients.

Methods: Patients (n = 160) scheduled for elective abdominal surgery were included in this prospective clinical study.