Hepatocyte isolation from resected benign tissues: Results of a 5-year experience.

Aim: To analyze retrospectively a 5-year experience of human hepatocyte isolation from resected liver tissues with benign disease.

Methods: We established a method of modified four-step retrograde perfusion to isolate primary human hepatocytes. Samples were collected from the resected livers of patients with intrahepatic duct calculi (n = 7) and liver hemangioma (n = 17).

Reversible immortalization of human hepatocytes mediated by retroviral transfer and site-specific recombination.

Aim: To establish a method for the reversible immortalization of human hepatocytes, which may offer a good and safe source of hepatocytes for practical applications.

Methods: We successfully isolated primary human hepatocytes from surgically resected liver tissue taken from a patient with liver hemangiomas. The freshly isolated cells were then immortalized with retroviral vector SSR#69 expressing simian virus 40 large T antigen (SV40T) and hygromycin-resistance genes flanked by paired loxP recombination targets.

[Practice and exploration of dynamic management of medical equipment].

Through-lifetime dynamic management, a new management model of medical equipment, is introduced in this paper. Relating to the situation of our hospital, we make a preliminary conference about theory and practice of the model from the aspects of the basement of software and hardware, primary way of practicing, and actual results.