[Preliminary Study of Lonicera hypoglauca on Germination Conditions of Sand Culture Seeds and Sterilization Method of Sand Culture Seedling Sterilization].

Objective: To explore the germination conditions of Lonicera hypoglauca sand culture seeds and the effects of sand culture seedlings sterilization.

Methods: 0.1% HgCl2 with different sterilization time, different illumination time and temperature culture condition were adopted to study the germination conditions of sand culture seeds.

[Determination of chlorogenic acid and luteoloside in lonicerae flos of different habitats and germplasms].

Objective: To establish an UPLC-PDA method for simultaneous determination of chlorogenic acid and luteoloside in Lonicerae Flos. On the basis of developed method, the quality of Lonicerae Flos from nine habitats and two local germplasms introduced from Qufu in Shandong to Wuming in Guangxi was evaluated.

Methods: The analysis was performed on a Waters Acquity UPLC H-Class system.

[The performance of agronomic traits in F1 crossing combinations derived from male sterile line of Platycodon grandiflorum].

Objective: To determine the possibility of heterosis utilization by male sterility in Platycodon grandiflorum.

Methods: The analysis of variance, heterosis over standard and correlation on 12 agronomic in 18 crossing combinations were carried out.

Results: Except the dry weight of upground part of plant, the length of main root and the diameter of middle part of the main root, all other agricultural traits were different significantly among the 18 F1 combinations.

[Preliminary study on the effect of climate factors on pollen fertility in Platycodon grandiflorum].

Objective: To have a better utilization of male sterile lines in heterozygotic breeding of Platycodon grandiflorum and provide theoretical basis for Platycodon grandiflorum hyboridization.

Methods: The pollen viability was detected by the means of aceto carmine dyeing, and the correlation analysis between climate factors of each anther development stage and pollen viability was estimated by Pearson coefficients.

Results: Pollen viability variation range of male-sterile line GP1BC1-12 was 0% - 27%.

[Heterosis analysis of the main agronomic characters of Platycodon grandiflorum].

Objective: Breeding the good varieties characters of Platycodon grandiflorum.

Methods: 20 combination, derived from 2 x 10 incomplete diallel cross with 2 male sterile lines and 10 four-generation inbred lines, analysis the 13 agronomic characters.

Results: The agronomic characters of F1 has obvious heterosis.

[Comparative study on O3/H2O2 and O3/Mn processes for removal of refractory organics in water].

A comparative study of O3/H2O2 and O3/Mn was made to evaluate their respective effectiveness and mechanism for removal of refractory organics from water with nitrobenzene as the model pollutant. It was found that both processes enhanced the degradation of nitrobenzene that can be hardly degraded by ozonation and had respective optimum dosage of H2O2 and Mn(II) under the experimental conditions. By comparing the mechanism of O3/H2O2 process and considering the chemical character of nitrobenzene and variation of residual aqueous ozone, it is speculated that the degradation of nitrobenzene by O3/Mn might follow a radical pathway.