Aptamer-Embedded Zirconium-Based Metal-Organic Framework Composites Prepared by De Novo Bio-Inspired Approach with Enhanced Biosensing for Detecting Trace Analytes.

A series of Zr-based metal-organic framework (MOF) composites embedded with three kinds of aptamer strands (509-MOF@Apt) were achieved by a one-step de novo synthetic approach. A platform for ultrasensitive detection of analytes, namely, thrombin, kanamycin, and carcinoembryonic antigen (CEA), was also established. Considering the conformational changes caused by the binding interactions between aptamer strands and targeted molecules, the label-free electrochemical aptasensors based on 509-MOF@Apt composites could be developed to detect various target molecules.

Pore modulation of zirconium-organic frameworks for high-efficiency detection of trace proteins.

Tunable electrochemical biosensors based on analogous Zr-MOFs were developed for protein detection, the performances of which rely on the pore sizes and surroundings of the MOFs that show diverse binding behaviors to aptamers and then the targeted proteins. The optimized Zr-MOF-based sensor has high selectivity to lysozyme in a wide concentration range and a low detection limit of 3.6 pg mL, with good repeatability, stability, and applicability in real samples.