[Effects of different factors on transformed hairy root in Gynostemm apentaphyllum].

Objective: To induce hairy roots of Gynostemm apentaphyllum by Agrobacterium rhizogenes strains.

Methods: Hairy roots were induced by the co-culture method of explants and Agrobacterium rhizogenes strains. Effects of different Agrobacterium rhizogenes strains, explants, pre(co)-culture time, Bacterial concentration, infecting time, As concentration and antibiotic medium on the transformation frequency were studied.