The study focuses on a specific gene, ApESR16, which encodes a 16 kDa protein in the Chinese oak silkworm, revealing its sequence, structure, and evolutionary relationships to other insect proteins.
ApESR16 is shown to have distinct cholesterol/lipid binding sites and possesses varying degrees of sequence identity with related proteins in both lepidopteran and non-lepidopteran insects, indicating significant evolutionary divergence.
Additionally, the expression of ApESR16 is influenced by the hormonal treatment of 20-hydroxyecdysone, suggesting its potential role in regulating diapause during the insect's developmental cycle.