10-Hydroxydec-2-enoic acid reduces vascular smooth muscle cell inflammation via interacting with Toll-like receptor 4.

Background: 10-Hydroxydec-2-enoic acid (10-HDA), a unique and marker compound in royal jelly, has a wide range of bio-activities. However, its role in regulating inflammation of vascular smooth muscle cell (VSMC), which is essential to a set of vascular diseases, is still unknown.

Purpose: Our study aimed to investigate whether 10-HDA exerts effect on VSMC inflammation via interacting with toll-like receptor 4 (TLR4), a pivotal inflammatory initiator.

Correlation Between Fecal Microbiota and Corticosteroid Responsiveness in Primary Immune Thrombocytopenia: an Exploratory Study.

Corticosteroids (CSs) are the initial therapy for immune thrombocytopenia (ITP); however, their efficacy is not adequately predicted. As a novel biomarker, the composition of the gut microbiota is non-invasively tested and altered in patients with ITP. This study aims to develop a predictive model that leverages gut microbiome data to predict the CS response in patients with ITP within the initial four weeks of treatment.

Transcriptomic analysis of Virescentia guangxiensis (Rhodophyta: Batrachospermales) revealed differential expression of genes in gametophyte and chantransia life phases.

Background: The genus Virescentia is a significant member of the Batrachospermaceae, exhibiting distinctive life history characteristics defined by alternating generations. This group of taxa has specific environmental requirements for growth. This paper investigates Virescentia, which primarily thrives in freshwater environments, such as streams and springs, characterized by low light, low temperatures, and high dissolved oxygen levels.

Multilayer analysis of ethnically diverse blood and urine biomarkers for breast cancer risk and prognosis.

Breast cancer (BC) is one of the most common malignancies among women globally, characterized by complex pathogenesis involving various biomarkers present in blood and urine. To enhance understanding of the genetic associations between biomarkers and BC via multidimensional, cross ethnic investigations. Based on GWAS data of 35 blood and urine biomarkers from European populations, we adopted multiple analysis strategies including univariable Mendelian randomization (MR) analysis, reverse MR analysis, sensitivity analysis and multivariate MR to identify potential biomarkers associated with BC risk and survival.

NOX4 modulates breast cancer progression through cancer cell metabolic reprogramming and CD8 T cell antitumor activity.

Introduction: Breast cancer is the most frequently diagnosed malignancy and a leading cause of cancer-related mortality among women worldwide. Although NADPH oxidase 4 (NOX4) has been implicated in various oncogenic processes, its exact function in breast cancer progression, metabolic reprogramming, and immune modulation remains unclear.

Methods: We used murine 4T1 and EO771 breast cancer models to generate NOX4 knockout (KO) cell lines via CRISPR/Cas9.

Mendelian randomization and colocalization analysis reveal the role of IL-6 signaling pathway in psoriasis and potential therapeutic targets.

IL-6 signaling is critical in the pathogenesis of several autoimmune diseases, including psoriasis and psoriatic arthritis. This study utilized Mendelian randomization (MR) analysis to investigate the genetic associations between IL-6 signaling, soluble IL-6 receptor (sIL-6R) levels, and psoriasis and its arthritis. Additionally, the therapeutic potential of IL-6R inhibitors was evaluated.

Exploring the potential antidepressant mechanisms of ibuprofen and celecoxib based on network pharmacology and molecular docking.

Background: Evidence has shown that ibuprofen and celecoxib are effective in improving depressive symptoms, but their mechanisms of action are unclear. In this study, we aimed to determine the relationship between these two drugs and depressive disorder (DD) and elucidate potential mechanisms of action.

Methods: Relevant targets for ibuprofen, celecoxib, and DD were obtained and screened from multiple online drug and disease public databases.

Evaluating the Efficacy of Large Language Models in Guiding Treatment Decisions for Pediatric Refractive Error.

Introduction: Effective management of pediatric myopia, which includes treatments like corrective lenses and low-dose atropine, requires accurate clinical decisions. However, the complexity of pediatric refractive data, such as variations in visual acuity, axial length, and patient-specific factors, pose challenges to determining optimal treatment. This study aims to evaluate the performance of three large language models in analyzing these refractive data.

Ca enhanced the wastewater treatment performance of microalgal-bacterial consortia: Response of extracellular polymeric substances and bacterial communities.

The technology of microalgae-bacteria consortia (MBC) for wastewater treatment is currently facing a variety of challenges. One of the main issues is the construction of structurally and functionally stable symbiont. Ca may be involved in this process, but the underlying mechanism is not well understood.

PGLYRP2 drives hepatocyte-intrinsic innate immunity by trapping and clearing hepatitis B virus.

Spontaneous clearance of hepatitis B virus (HBV) is frequent in adults (95%) but rare in infants (5%), emphasizing the critical role of age-related hepatic immunocompetence. However, the underlying mechanisms of hepatocyte-specific immunosurveillance and age-dependent HBV clearance remain unclear. Here, we identified PGLYRP2 as a hepatocyte-specific pattern recognition receptor with age-dependent expression, and demonstrated that phase separation of PGLYRP2 was a critical driver of spontaneous HBV clearance in hepatocytes.

Risk factors and clinical outcomes of cytomegalovirus infection following haploidentical hematopoietic stem cell transplantation in patients with aplastic anemia: a single-center retrospective study.

Background: Cytomegalovirus (CMV) infection remains a critical cause of mortality after allogeneic hematopoietic stem cell transplantation, despite significant advancements in CMV prevention and treatment with the introduction and widespread use of letermovir. However, in China, due to limitations in the availability and cost of medications, some patients still face challenges in accessing letermovir. For this subset of the population, exploring the risk factors for CMV infection remains significant in predicting its occurrence.

Incidence, outcome and risk factors of perioperative pulmonary complications in pediatric patients with mediastinal mass.

Purpose: This retrospective study was designed to identify the incidence, outcome and analyze risk factors of perioperative pulmonary complications in children with mediastinal mass so that to achieve the Enhanced Recovery After Surgery(ERAS).

Methods: This study enrolled 247 pediatric patients undergoing mediastinal mass resection from June 2016 to June 2022. Medical record was reviewed for basic characteristics, presenting symptoms, preoperative imaging results, intraoperative data, and the occurrence of any pulmonary complications.

DRP1: shedding light on the complex nexus of mitochondrial fission and breast cancer.

Breast cancer (BC) is a global women's health concern, with ongoing research to address diagnostic and treatment challenges. Understanding the developmental mechanisms is vital for improved clinical prevention and treatment strategies. Mitochondria, undergo dynamic processes like fission and fusion, regulated by proteins like Dynamic-related protein 1 (DRP1).

Initial assessment of PNS safety for interventionalists during image-guided procedures.

Purpose: This study investigates peripheral nerve stimulation (PNS) safety thresholds for health professionals performing MRI procedures and the variation of arm rotations in close vicinity to the magnet bore.

Methods: Employing two posable human body models, this research utilized quasi-static electromagnetic calculations and neurodynamic simulations to assess PNS thresholds. Different arm rotations are compared for standing interventionalist's posture assuming the supine patient position, typical for medical interventions inside MRI devices.

Hepatic fibroblast growth factor 21 is required for curcumin or resveratrol in exerting their metabolic beneficial effect in male mice.

Background: Our mechanistic understanding on metabolic beneficial effects of dietary polyphenols has been hampered for decades due to the lack of functional receptors for those compounds and their extremely low plasma concentrations. Recent studies by our team and others suggest that those dietary polyphenols target gut microbiome, and gut-liver axis and that hepatic fibroblast factor 21 (FGF21) serves as a common target for various dietary interventions.

Methods: Utilizing liver-specific FGF21 null mice (lFgf21), we are asking a straightforward question: Is hepatic FGF21 required for curcumin or resveratrol, two typical dietary polyphenols, in exerting their metabolic beneficial effect in obesogenic diet-induced obesity mouse models.

Flavonoids of Pigmented Rice Lower Blood Glucose Levels by Regulating the Glucose Metabolism Pathway and Gut Microbiota Composition.

Black and red rice have many physiological effects, including antioxidant capacity and decreasing blood glucose, because they are flavonoid-rich. Here, our study found that Hongcaomi (a red rice variety) of five examined rice cultivars contained the highest total polyphenol content and total flavonoid content and the strongest antioxidant activity, with values of 5.83 mg GAE/g, 1.

Differentiations in Illness Duration, Thyroid-Stimulating Hormone, Glucose and P300 Latency Between Drug-Naïve Unipolar and Bipolar Depression: A Comparative Cross-Sectional Study.

Background: Distinguishing bipolar depression (BD) from unipolar depression (UD) remains a major clinical challenge, especially in drug-naïve patients. The present study aimed to investigate whether demographic, clinical, and biochemical parameters can help differentiate drug-naïve BD from UD.

Methods: Drug-naïve patients with UD and BD were recruited from Shandong Mental Health Center.

Optimizing ultrasonic extraction of polysaccharides from Spirulina platensis and evaluating their antioxidant and antibacterial activities in acidic environments.

In this study, the response surface method was used to optimize the ultrasonic extraction of polysaccharide from Spirulina platensis. The optimal extraction conditions were ultrasonic time 50 min, solid-liquid ratio 1: 40, ultrasonic power 265 w, and the predicted extraction rate was 6.861 ± 0.

Forsythiaside A Ameliorates Inflammation by Regulating the Autophagy in Methotrexate-induced Intestinal Mucositis.

Background: Methotrexate (MTX) effectively eliminates cancerous cells but can also cause inflammation intestinal, known as mucositis. Forsythiaside A (FTA) from Forsythia suspensa has shown promise in relieving mucositis by targeting the NLRP3 pathways. Since NLRP3 inflammasome activation is negatively regulated by autophagy, this study explores how FTAmediated autophagy affects NLRP3 inflammasome in treating MTX-induced intestinal inflammation.

Hematopoietic stem cell transplantation and immunosuppressive therapy: implications of clonal haematopoiesis.

Aplastic anemia (AA) is a life-threatening bone marrow failure syndrome. The advent of next-generation sequencing (NGS) has shed light on the link between somatic mutations (SM) and the efficacy of immunosuppressive therapy (IST) in AA patients. However, the relationship between SM and hematopoietic stem cell transplantation (HSCT) has not been extensively explored.

Expression and characterization of the complete cyanophage genome PP in the heterologous host Synechococcus elongatus PCC 7942.

In this study, we successfully integrated the full-length genome of the cyanophage PP into the non-host cyanobacterium Synechococcus elongatus PCC 7942, facilitated by conjugation via Escherichia coli. To address the challenge posed by the toxic open reading frames (ORFs) of PP in E. coli, we first identified and characterized three toxic ORFs.

and activity of 1,2,3,4,6-O-pentagalloyl-glucose against .

Invasive fungal infections have become an increasingly serious threat to global human health, underscoring the urgent need for the development of new antifungal drugs. In this study, we found a natural polyphenolic compound 1,2,3,4,6-O-pentagalloyl-glucose (PGG), which is present in various plants and herbs. PGG showed broad-spectrum antifungal activities, enhancing the efficacy of fluconazole.

Urinary phthalate metabolites associated with increased prevalence of gallstone disease in U.S. adults: data from the NHANES study.

Background: Phthalate exposure has been hypothesized to influence cholesterol metabolism and gallstone pathogenesis, but previous studies are limited. We aimed to examine the associations between urinary phthalate metabolites and prevalence of gallstone disease in a nationally representative sample.

Methods: We analyzed data on 1,696 adults aged ≥ 30 years from the National Health and Nutrition Examination Survey (NHANES) 2017-2018.

Structure characterization and preliminary immune activity of a glucomannan purified from Allii Tuberosi Semen.

A novel glucomannan, named ATSW-1, was obtained from Allii Tuberosi Semen by ion and gel permeation chromatography purification. Its structure was characterized using high-performance chromatography, Fourier transform infrared spectroscopy, gas chromatography-mass spectrometry, and nuclear magnetic resonance spectroscopy. The in vitro immune activities of ATSW-1 were examined using the Cell Counting Kit-8, the neutral red phagocytosis assay, and the secretion of related cytokines.