Production of an amylase from newly Bacillus strain: Optimization by response-surface methodology, characterization and application with a fungal lipase in bread making.

Using the statistical approaches, the optimization of amylase production by a newly Bacillus strain was achieved. Keeping the insignificant factor at lower levels and following the optimized condition (inoculum size 0.3; 19 h of incubation; 18 g L of starch; 4 g L of MgSO; and 15 g L NaCl) the amylase activity has reached 18.

Impact of missense mutations on the structure-function relationship of human succinyl-CoA synthetase using in silico analysis.

The encephalomyopathic mtDNA depletion syndrome with methylmalonic aciduria is associated with succinyl-CoA synthetase (SCS) deficiency caused by pathogenic variants in genes encoding its two subunits. SCS is a mitochondrial enzyme involved in several metabolic pathways and acts as a heterodimer composed of α and β subunits encoded by SUCLG1 and SUCLA2 genes, respectively. The purpose of this study was to analyze the effects of the most pathogenic non-synonymous single nucleotide polymorphisms (nsSNPs) by applying, using different prediction tools, a filtering strategy, on the 343 and 365 nsSNPs found in SUCLG1 and SUCLA2 genes, respectively, retrieved from the databases, then to evaluate their structural and functional effects using homology modeling and molecular docking.

Optimization of newly isolated α-amylase production using orange peels and crab shells and application in wastewater treatment.

A newly isolated amylolytic strain was identified as based on 16S and 16-23S gene sequencing (Accession numbers OP811441.1 and OP819558, respectively), optimization strategies, using one variable at time (OVAT) and Plackett-Burman design, were employed to improve the alpha-amylase (α-amylase) production. Condition inferred revealed that the optimal physical parameters for maximum enzyme production were 30 °C, pH 7.

Expression and characterization of an organic solvent tolerant recombinant lipase from SH6 for food wastewater treatment.

The study illustrated here aims on an organic solvent tolerant lipase from (SCL). The gene part, encoding the mature lipase, was cloned and sequenced. The concluded polypeptide sequence, equivalent to the protein, consist of 388 amino acid residues with a molecular mass of about 45 kDa.

A novel homozygous PIGO mutation associated with severe infantile epileptic encephalopathy, profound developmental delay and psychomotor retardation: structural and 3D modelling investigations and genotype-phenotype correlation.

The PIGO gene encodes the GPI-ethanolamine phosphate transferase 3, which is crucial for the final synthetic step of the glycosylphosphatidylinositol-anchor serving to attach various proteins to their cell surface. These proteins are intrinsic for normal neuronal and embryonic development. In the current research work, a clinical investigation was conducted on a patient from a consanguineous family suffering from epileptic encephalopathy, characterized by severe seizures, developmental delay, hypotonia, ataxia and hyperphosphatasia.

Optimization using response surface methodology of phospholipase C production from suitable for soybean oil degumming.

This work deals with the optimization of an extracellular phospholipase C production by (PLC) using Response Surface Methodology (RMS) and Box-Behnken design. In fact, after optimization, a maximum phospholipase activity (51 U/ml) was obtained after 6 h of cultivation on tryptone (10 g/L), yeast extract (10 g/L), NaCl (8.125 g/L), pH 7.

Spinal radiographic progression is correlated with preclinical atherosclerosis in spondyloarthritis.

Background: A higher prevalence of cardiovascular risk was observed in spondyloarthritis (SpA). The relationship between disease-related factors structural damage and subclinical atherosclerosis is still unknown.

Objective: The aim of our study was to evaluate the association of subclinical atherosclerosis with radiographic structural damage in patients with SpA.

Structure elucidation of lipase. Molecular dynamics simulations to investigate the effects of calcium and zinc ions on the structural stability.

Cold-adapted and organic solvent tolerant lipases have significant potential in a wide range of synthetic reactions in industry. But there are no sufficient studies on how these enzymes interacts with their substrates. Herein, the predicted structure and function of the lipase (SCL) are studied.

The response surface methodology for optimization of sp. C2SS100 lipase immobilization onto CaCO for treatment of tuna wash processing wastewater.

An immobilized enzyme could exhibit selectively modified physicochemical properties, and it might offer a better environment for the enzyme activity. In this study, the immobilization yield of crude sp. lipase was optimized to improve its stability.

Fungal lipases as biocatalysts: A promising platform in several industrial biotechnology applications.

Many researchers have found fungi as a reliable source of lipase due to the versatility of their properties, ease of mass production, thermal stability, pH stability, broad substrate specificity, retained activity in organic solvents, and their low-cost extraction procedure. This review paper presents an overview about the main aspects of fungal lipases screened from several types of strains as well as their use as biocatalysts. Additionally, some biochemical properties will be reported.

Endothelial dysfunction and increased carotid intima-media thickness in patients with spondyloarthritis without traditional cardiovascular risk factors.

Background: The aim of our study was to assess subclinical atherosclerosis in spondyloarthritis (SpA) by combining three ultrasound methods (flow-mediated dilation (FMD), carotid intima-media thickness (cIMT) and Ankle Brachial Index (ABI)) and to determine the predictive factors of theses parameters.

Methods: This was a case control study conducted over 12 months including 47 patients with SpA-free-cardiovascular (CV) disease in comparison with age and sex matched 47 healthy controls. Sociodemographic, clinical and biological features as well as therapeutic modalities were recorded in our patients.

Increased epicardial adipose tissue thickness correlates with endothelial dysfunction in spondyloarthritis.

Introduction: We aimed to investigate the relationship between epicardial adipose tissue (EAT) thickness, flow-mediated dilation (FMD), and carotid intima-media thickness (cIMT) in spondyloarthritis (SpA) patients compared to healthy controls.

Methods: We performed a cross-sectional study including SpA patients aged ≤ 50 years without traditional cardiovascular risk factors and healthy controls matched for age and gender. Baseline characteristics, laboratory data, and SpA-related parameters were recorded.

Optimization of an organic solvent-tolerant lipase production by SH6. Immobilization for biodiesel production and biodegradation of waste greases.

Using the statistical approach, this work seeks to optimize the process parameters to boost the generation of an organic solvent-tolerant lipase by SH6. The main parameters influencing the enzyme production were identified by using Plackett-Burman's screening design. Among the test variables, only tryptone (25 g/L), malt extract (2.

Dissecting the Interaction Deficiency of a Cartilaginous Fish Digestive Lipase with Pancreatic Colipase: Biochemical and Structural Insights.

A full-length cDNA encoding digestive lipase (SmDL) was cloned from the pancreas of the smooth-hound (). The obtained cDNA was 1350 bp long encoding 451 amino acids. The deduced amino acid sequence has high similarity with known pancreatic lipases.

Intestinal phospholipase A from Sparidae species: Functional properties and cytotoxic potential evaluation.

Marine species have gained significant attention as potential source for a broad spectrum of bioactive proteins. Fish phospholipases A (PLA) have attracted renewed interest due to their excellent properties in lipid digestion. Herein, we report for the first time the catalytic properties of two intestinal secreted PLA (sPLA) identified from Diplodus sargus (IDsPLA) and Sparus aurata (ISaPLA).

Studies of crab digestive phospholipase acting on phospholipid monolayers: Activation by temperature.

Secreted phospholipases A2 (sPLA2) are water-soluble lipolytic enzymes that act at the interface of organized lipid substrates, where the catalytic step is coupled to various interfacial phenomena as enzyme penetration, solubilisation of reaction products, lateral packing and loss of mechanical stability of organized assemblies of phospholipid molecule, among others. Using the monomolecular film technique, we compared the interfacial properties of crab digestive sPLA (CDPL) with those of the porcine pancreatic one (PPPL). A kinetic study on the surface pressure dependency of the two sPLA was performed using monomolecular films of three different substrates: di C-PC (1.

Measurement System for Lossy Capacitive Sensors: Application to Edible Oils Quality Assessment.

This paper aimed to develop a portable, low-cost, and easy-to-use measurement system for oil quality degradation assessment. The main two chemical parameters affected by frying are the total polar compounds (TPC) and free fatty acids. The system should characterize the change of chemical parameters by measuring the changes in its dielectric parameters.

Purification and biochemical characterization of an organic solvent-tolerant and detergent-stable lipase from Staphylococcus capitis.

A mesophilic bacterial culture, producing an extracellular alkaline lipase, was isolated from the gas-washing wastewaters generated from the Sfax phosphate plant of the Tunisian Chemical Group and identified as Staphylococcus capitis strain. The lipase, named S. capitis lipase (SCL), has been purified to homogeneity from the culture medium.

Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A.

In this study, we have produced for the first time a fish phospholipase (PLA) in heterologous system (E. coli). The Diplodus annularis PLA (DaPLA) was then refolded from inclusion bodies and purified by Ni-affinity chromatography.

Synergistic effect of polysaccharides, betalain pigment and phenolic compounds of red prickly pear (Opuntia stricta) in the stabilization of salami.

The aim of this work is to try to substitute some synthetic additives by a natural extract from red prickly pear (Opuntia stricta) which known by its richness on bioactive polysaccharides mainly consisting of galactose, rhamnose and galacturonic acid. This natural fruit has a high content of carbohydrates above 18.81% FM.

Efficient heterologous expression, functional characterization and molecular modeling of annular seabream digestive phospholipase A.

Here we report the cDNA cloning of a phospholipase A (PLA) from five Sparidae species. The deduced amino acid sequences show high similarity with pancreatic PLA. In addition, a phylogenetic tree derived from alignment of various available sequences revealed that Sparidae PLA are closer to avian PLA group IB than to mammals' ones.

The smooth-hound lipolytic system: Biochemical characterization of a purified digestive lipase, lipid profile and in vitro oil digestibility.

In order to identify fish enzymes displaying novel biochemical properties, we choose the common smooth-hound (Mustelus mustelus) as a starting biological material to characterize the digestive lipid hydrolyzing enzyme. A smooth-hound digestive lipase (SmDL) was purified from a delipidated pancreatic powder. The SmDL molecular weight was around 50kDa.

Functional and Structural Characterization of a Thermostable Phospholipase A from a Sparidae Fish (Diplodus annularis).

Novel phospholipase (PLA) genes from the Sparidae family were cloned. The sequenced PLA revealed an identity with pancreatic PLA group IB. To better understand the structure/function relationships of these enzymes and their evolution, the Diplodus annularis PLA (DaPLA) was overexpressed in E.

Biochemical characterization, cloning and molecular modeling of a digestive lipase from red seabream (): Structural explanation of the interaction deficiency with colipase and lipidic interface.

Red seabream digestive lipase (RsDL) was purified from fresh pyloric caeca. Pure RsDL has an apparent molecular mass of 50 kDa. The RsDL is more active on short-chain triacylglycerols (TC), and enzymatic activity decreases when medium (TC) or long-chain (olive oil) triacylglycerols were used as substrates.

Biochemical and molecular characterization of a lipase from an Algerian isolated Staphylococcus aureus strain.

A Staphylococcus aureus strain, isolated from an Algerian biotope, secretes a non-induced lipase in the culture medium. The S. aureus lipase (SAL) was purified to homogeneity.