Comparative genome mining and heterologous expression of an orphan NRPS gene cluster direct the production of ashimides.

The ever-increasing bacterial genomic repositories reveal a great number of uncharacterized biosynthetic gene clusters, representing a tremendous resource for natural product discovery. Genome mining of the marine sp. NA03103 indicates the presence of an orphan nonribosomal peptide synthetase (NRPS) gene cluster (), to which there are no homologous gene clusters in the public genome databases.

Amycolamycins A and B, Two Enediyne-Derived Compounds from a Locust-Associated Actinomycete.

Two novel enediyne-derived natural products, amycolamycins A and B (1 and 2), were characterized from a locust-associated actinomycete Amycolatopsis sp. HCa4. Amycolamycins A and B contain a unique 2-(cyclopenta[a]inden-5-yl)oxirane core with suspected enediyne polyketide biosynthetic origin.