Publications by authors named "Felix Rodriguez-Juarez"
Article Synopsis
- The research investigates whether the gene expression profile (transcriptome) of cumulus cells can predict the developmental potential of embryos derived from donor oocytes.
- Cumulus cells were analyzed using RNA sequencing from three groups based on oocyte developmental outcomes: unable to reach the blastocyst stage, able but not resulting in pregnancy, and those that did result in a clinical pregnancy.
- Results showed minimal differences in gene expression across groups, with only a few specific genes linked to developmental potential, suggesting cumulus cell transcriptome is not a strong indicator of successful pregnancy establishment.
Nitric oxide (NO) inhibits mitochondrial respiration by decreasing the apparent affinity of cytochrome c oxidase (CcO) for oxygen. Using iNOS-transfected HEK 293 cells to achieve regulated intracellular NO production, we determined NO and O(2) concentrations and mitochondrial O(2) consumption by high-resolution respirometry over a range of O(2) concentrations down to nanomolar. Inhibition of respiration by NO was reversible, and complete NO removal recovered cell respiration above its routine reference values.
View Article and Find Full Text PDFNitric oxide (NO) is a widespread biological messenger that has many physiological and pathophysiological roles. Most of the physiological actions of NO are mediated through the activation of sGC (soluble guanylate cyclase) and the subsequent production of cGMP. NO also binds to the binuclear centre of COX (cytochrome c oxidase) and inhibits mitochondrial respiration in competition with oxygen and in a reversible manner.
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