Whole-Genome Sequence of sp. , Isolated from Riccia fluitans.

Here, we present the Nanopore-only genome sequence of sp. . It was isolated from Riccia fluitans ecotype BoGa-3 and its source was Botanical Garden Osnabrück (Germany).

Developmental Plasticity of the Amphibious Liverwort .

The colonization of land by ancestors of embryophyte plants was one of the most significant evolutionary events in the history of life on earth. The lack of a buffering aquatic environment necessitated adaptations for coping with novel abiotic challenges, particularly high light intensities and desiccation as well as the formation of novel anchoring structures. Bryophytes mark the transition from freshwater to terrestrial habitats and form adaptive features such as rhizoids for soil contact and water uptake, devices for gas exchange along with protective and repellent surface layers.

Gamete expression of TALE class HD genes activates the diploid sporophyte program in .

Eukaryotic life cycles alternate between haploid and diploid phases and in phylogenetically diverse unicellular eukaryotes, expression of paralogous homeodomain genes in gametes primes the haploid-to-diploid transition. In the unicellular chlorophyte alga KNOX and BELL TALE-homeodomain genes mediate this transition. We demonstrate that in the liverwort paternal (sperm) expression of three of five phylogenetically diverse BELL genes, Mp, and maternal (egg) expression of both Mp and Mp mediate the haploid-to-diploid transition.

Transformation of , an Amphibious Liverwort Dynamically Responding to Environmental Changes.

The colonization of land by streptophyte algae, ancestors of embryophyte plants, was a fundamental event in the history of life on earth. Bryophytes are early diversifying land plants that mark the transition from freshwater to terrestrial ecosystems. The amphibious liverwort can thrive in aquatic and terrestrial environments and thus represents an ideal organism to investigate this major transition.

Insights into Land Plant Evolution Garnered from the Marchantia polymorpha Genome.

The evolution of land flora transformed the terrestrial environment. Land plants evolved from an ancestral charophycean alga from which they inherited developmental, biochemical, and cell biological attributes. Additional biochemical and physiological adaptations to land, and a life cycle with an alternation between multicellular haploid and diploid generations that facilitated efficient dispersal of desiccation tolerant spores, evolved in the ancestral land plant.

TALEN-mediated genome-editing approaches in the liverwort yield high efficiencies for targeted mutagenesis.

Background: The liverwort occupies a crucial position in land plant evolution and provides the opportunity to investigate adaptations to a terrestrial plant life style. Marchantia reverse genetic analyses have thus far been conducted by employing a homologous recombination approach, which yields an efficiency of around 3%. Availability of the characterized and suitable endogenous Mp promoter prompted us to establish the TALEN gene targeting technique for Marchantia.

Identification of miRNAs and Their Targets in the Liverwort Marchantia polymorpha by Integrating RNA-Seq and Degradome Analyses.

Bryophytes (liverworts, hornworts and mosses) comprise the three earliest diverging lineages of land plants (embryophytes). Marchantia polymorpha, a complex thalloid Marchantiopsida liverwort that has been developed into a model genetic system, occupies a key phylogenetic position. Therefore, M.

Optical measurement of dimensional parameters of the breast with subjects in prone position.

Various applications require information on breast parameters, such as breast length and volume. An optical system was designed and tested for measuring these parameters with subjects in a prone position. The study results were used for optimizing patient positioning and handling for a future breast computed tomography (BCT) system.

Comparison of the MpEF1α and CaMV35 promoters for application in Marchantia polymorpha overexpression studies.

Constitutive promoters are essential tools for analyses of gene functions by transgenic approaches. For overexpression and silencing studies of genes, a ubiquitous and strong expression of genes under investigation as well as selection markers is preferred. For future applications in the emerging basal plant model system Marchantia polymorpha, a liverwort, activities of the viral 35S cauliflower mosaic virus promoter and the endogenous elongation factor 1α (MpEF1α) promoter were analyzed.

Time-delayed summation as a means of improving resolution on fast rotating computed tomography systems.

Purpose: Modern computed tomography (CT) systems are supporting increasingly fast rotation speeds, which are a prerequisite for fast dynamic acquisition, e.g. in perfusion imaging, and for new modalities such as dedicated breast CT, where breathhold scanning is indicated.