Establishing reliable DNA barcoding primers for jumping plant lice (Psylloidea, Hemiptera).

Objectives: DNA Barcoding has proven to be a reliable method for rapid insect identification. The success of this method is based on the amplification of a specific region, the 'Folmer' barcode region at the 5´ start of the cytochrome c oxidase 1 gene (cox1), with universal primers. Previous studies showed failures of standard "universal" primers to amplify this region in psyllids.

Use of marine debris as nest material by ospreys.

The use of anthropogenic debris as nest-building materials may affect nest function. We study ospreys (Pandion haliaetus) on an island with scarce vegetation and high availability of beached marine debris. We describe the anthropogenic debris in osprey nests, evaluate the factors affecting its prevalence and abundance, and test its potential effects on breeding parameters.

Fungal Pathogens Associated with Aerial Symptoms of Avocado ( Mill.) in Tenerife (Canary Islands, Spain) Focused on Species of the Family Botryosphaeriaceae.

Fungi of the family Botryosphaeriaceae are considered responsible for various symptoms in avocado such as dieback, external necrosis of branches and inflorescences, cankers on branches and trunks, or stem-end rot of fruits. In recent years, these problems are becoming more frequent in avocado orchards in the Canary Islands (Spain). This work includes the characterization of fungal species involved in these diseases, which were isolated from avocado crops in Tenerife Island between 2018 and 2022.

Genomic basis of insularity and ecological divergence in barn owls (Tyto alba) of the Canary Islands.

Islands, and the particular organisms that populate them, have long fascinated biologists. Due to their isolation, islands offer unique opportunities to study the effect of neutral and adaptive mechanisms in determining genomic and phenotypical divergence. In the Canary Islands, an archipelago rich in endemics, the barn owl (Tyto alba), present in all the islands, is thought to have diverged into a subspecies (T.

Assessment of Psyllid Handling and DNA Extraction Methods in the Detection of ' Liberibacter Solanacearum' by qPCR.

' Liberibacter solanacearum' (CaLsol) is an uncultured bacterium, transmitted by psyllids and associated with several diseases in and crops. CaLsol detection in psyllids often requires insect destruction, preventing a subsequent morphological identification. In this work, we have assessed the influence on the detection of CaLsol by PCR in (Hemiptera: Psyllidae), of four specimen preparations (entire body, ground, cut-off head, and punctured abdomen) and seven DNA extraction methods (PBS suspension, squashing on membrane, CTAB, Chelex, TRIsure, HotSHOT, and DNeasy).

Landscape and Climatic Variations Shaped Secondary Contacts amid Barn Owls of the Western Palearctic.

The combined actions of climatic variations and landscape barriers shape the history of natural populations. When organisms follow their shifting niches, obstacles in the landscape can lead to the splitting of populations, on which evolution will then act independently. When two such populations are reunited, secondary contact occurs in a broad range of admixture patterns, from narrow hybrid zones to the complete dissolution of lineages.

Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Liberibacter Solanacearum from Different Host Plants in Spain.

is a bacterial group causing different diseases and disorders in plants. Among liberibacters, Liberibacter solanaceraum (CLso) produces disorders in several species mainly within Apiaceae and Solanaceae families. CLso isolates are usually grouped in defined haplotypes according to single nucleotide polymorphisms in genes associated with ribosomal elements.

' Liberibacter Solanacearum' Is Unlikely to Be Transmitted Spontaneously from Infected Carrot Plants to Citrus Plants by .

Bacteria belonging to ' Liberibacter spp.' are associated with various severe diseases in the five continents. The African citrus psyllid (Hemiptera: Triozidae) is an efficient vector of citrus huanglongbing-HLB disease, absent in the Mediterranean basin.

Survey in Nurseries and Irrigation Water Reservoirs as Sources of Oomycetes Found in Avocado Orchards in the Canary Islands.

Nursery stocks and irrigation water are important sources of spp. In this study, 20 irrigation water reservoirs and 10 avocado nurseries were surveyed in the Canary Islands between 2013 and 2015 to investigate their potential role in disseminating pathogenic species in avocado orchards. was isolated from one of the irrigation reservoirs, whereas, in two surveys conducted in nurseries, , the primary pathogen in avocado, was detected in addition to other species (, , , , and ) and The species recovered from nurseries were isolated mostly from propagated plants but also from nursery irrigation water, soil used for substrate preparation, and soil samples collected in orchards that supply seeds for seedling propagation.

Factors affecting the spatial distribution and breeding habitat of an insular cliff-nesting raptor community.

The specific spatial distribution and habitat association-strongly influenced by environmental factors or competitive interactions-are major issues in ecology and conservation. We located and georeferenced nesting sites of five cliff-nesting raptors (Egyptian vulture [a locally extinct species], common buzzard , osprey , common kestrel , Barbary falcon ), and common raven on one of the most biodiverse hotspot within the Canary Islands (Teno, Tenerife). We used generalized linear models to evaluate the factors affecting abundance, richness, and intra- and interspecific interactions.