Potentiation of antimicrobial photodynamic therapy with potassium iodide and methylene blue: targeting oral biofilm viability.

The study aimed to assess the impact of combining potassium iodide (KI) with methylene blue (MB) in antimicrobial photodynamic therapy (aPDT) within an oral biofilm formed in situ. A single-phase, 14 days in situ study involved 21 volunteers, who wore a palatal appliance with 8 bovine dentin slabs. These slabs were exposed to a 20% sucrose solution 8 times a day, simulating a high cariogenic challenge.

Reduction of microorganisms in carious dentin by photodynamic therapy mediated by potassium iodide added to methylene blue and red laser.

Objective: To evaluate the effect of the association of potassium iodide to antimicrobial photodynamic therapy on human carious dentin produced with a microcosm biofilm model.

Methods: A microcosm biofilm model was used to generate a caries lesion on human dentin. Pooled human saliva diluted with glycerol was used as an inoculum on specimens immersed on McBain artificial saliva enriched with 1 % sucrose (24 h at 37 °C in 5 % CO).

Does potassium iodide help in the microbial reduction of oral microcosm biofilms after photodynamic therapy with methylene blue and red laser?

Objective: To evaluate the efficacy of methylene blue (MB)-mediated antimicrobial photodynamic therapy (aPDT) doped with potassium iodide (KI) against oral microcosms biofilms cultured in dentin.

Methods: A saliva-glycerol stock formed from pooled human saliva was diluted in McBain artificial saliva with 1% sucrose (1:1), inoculated on bovine dentin blocks, and refreshed daily for 5 days. The biofilms were divided (n = 9/group) and treated with 0.