Biofilm formation and cell viability on monolithic zirconia with silver-doped sodalime glass.

The present study evaluated the effect of glass application with and without silver-doped soda-lime glass on roughness, biofilm formation, cell viability and flexural strength of a zirconia. Samples of 3-YTZP (3 mol% yttria stabilized tetragonal zirconia polycrystals) were divided into: polished (P); glaze (G); glass infiltration (INF); 4% silver-doped soda-lime glass (Ag4); glass infiltration + 4% silver-doped soda-lime glass (INF-Ag4); 5% silver-doped soda-lime glass (Ag5); glass infiltration + 5% silver-doped soda-lime glass (INF-Ag5). Samples were submitted to the following analyses: roughness (Ra); free surface energy (FSE); colony-forming units count (log CFU/mL); scanning electron microscopy (SEM); cytotoxicity (MTT assay) and flexural strength.

Probiotic Effects of Lactobacillus paracasei 28.4 to Inhibit Streptococcus mutans in a Gellan-Based Formulation.

Streptococcus mutans is considered to be a major bacterium involved in dental caries, and the control of virulence mechanisms is fundamental to prevent disease. Probiotics present a promising preventive method; however, the use of probiotics requires its incorporation into delivery materials to facilitate oral colonization. Thus, we performed a comprehensive study examining preventive effects of Lactobacillus paracasei 28.

Secreted Products Inhibit Induced Oral Candidiasis.

In the oral cavity, species form mixed biofilms with , a pathogenic bacterium that can secrete molecules with antifungal activity. In this study, we extracted and fractioned culture filtrate of , seeking antifungal agents capable of inhibiting the biofilms, filamentation, and candidiasis by . Active UA159 supernatant filtrate components were extracted via liquid-liquid partition and fractionated on a C-18 silica column to resolve fraction 1 (SM-F1) and fraction 2 (SM-F2).

Anidulafungin liposome nanoparticles exhibit antifungal activity against planktonic and biofilm Candida albicans.

Fungal infections can cause significant patient morbidity and mortality. Nanoparticle therapeutics have the potential to improve treatment of these infections. Here we report the development of liposomal nanoparticles incorporating anidulafungin, a potent antifungal, with the goal of increasing its solubility and aiding in localization to fungi.

Effects of surface pre-reacted glass-ionomer (S-PRG) eluate on spp.: antifungal activity, anti-biofilm properties, and protective effects on against infection.

Surface pre-reacted glass-ionomer (S-PRG) is a bioactive filler produced by PRG technology, which is applied to various dental materials. The inhibitory effects of S-PRG eluate against , the most common fungal oral pathogen, were investigated. Minimum inhibitory concentrations (MIC) and anti-biofilm activities were tested against , , , and .

Anti- Activity of Thiazolylhydrazone Derivatives in Invertebrate and Murine Models.

Candidiasis is an opportunistic fungal infection with being the most frequently isolated species. Treatment of these infections is challenging due to resistance that can develop during therapy, and the limited number of available antifungal compounds. Given this situation, the aim of this study was to evaluate the antifungal activity of four thiazolylhydrazone compounds against .

Temporal Profile of Biofilm Formation, Gene Expression and Virulence Analysis in Candida albicans Strains.

The characterization of Candida albicans strains with different degrees of virulence became very useful to understand the mechanisms of fungal virulence. Then, the objective of this study was to assess and compare the temporal profiles of biofilms formation, gene expression of ALS1, ALS3, HWP1, BCR1, EFG1, TEC1, SAP5, PLB2 and LIP9 and virulence in Galleria mellonella of C. albicans ATCC18804 and a clinical sample isolated from an HIV-positive patient (CA60).

Action of antimicrobial photodynamic therapy on heterotypic biofilm: Candida albicans and Bacillus atrophaeus.

The increase in survival and resistance of microorganisms organized in biofilms demonstrates the need for new studies to develop therapies able to break this barrier, such as photodynamic therapy, which is characterized as an alternative, effective, and non-invasive treatment. The objective was to evaluate in vitro the effect of antimicrobial photodynamic therapy on heterotypic biofilms of Candida albicans and Bacillus atrophaeus using rose bengal (12.5 μM) and light-emitting diode (LED) (532 nm and 16.

Influence of Candida krusei and Candida glabrata on Candida albicans gene expression in in vitro biofilms.

Objective: The present study aimed to evaluate the interactions between the species Candida albicans, Candida krusei and Candida glabrata in monotypic and mixed biofilm models formed in vitro as well as the relative expression of the ALS1, ALS3, HWP1, BCR1, EFG1, TEC1, SAP5, PLB2 and LIP9 genes.

Material And Methods: Mixed (C. albicans/C.