The temperature dependent dynamics and periodicity of dropwise condensation on surfaces with wetting heterogeneities.

Hypothesis: Biphilic surfaces, namely surfaces comprising hydrophilic areas with a (super)hydrophobic background, are used in nature and engineering for controlled dropwise condensation and liquid transport. These, however, are highly dependent on the surface temperature and subcooling.

Experiments: Here, biphilic surfaces were cooled inside a rotatable environmental chamber under controlled humidity.

Routes to turbulence in Taylor-Couette flow.

Fluid flows between rotating concentric cylinders exhibit two distinct routes to turbulence. In flows dominated by inner-cylinder rotation, a sequence of linear instabilities leads to temporally chaotic dynamics as the rotation speed is increased. The resulting flow patterns occupy the whole system and sequentially lose spatial symmetry and coherence in the transition process.

Inflammatory arthritis in systemic sclerosis is associated with elevated C-reactive protein and requires musculoskeletal ultrasound for reliable detection.

Objectives: About 25% of patients with systemic sclerosis (SSc) have elevated C-reactive protein (CRP) levels. Specific causes of CRP elevation are unknown so far. We aimed to investigate whether inflammatory arthritis is associated with CRP elevation.

A whole genome sequencing approach to anterior cruciate ligament rupture-a twin study in two unrelated families.

Predisposition to anterior cruciate ligament (ACL) rupture is multi-factorial, with variation in the genome considered a key intrinsic risk factor. Most implicated loci have been identified from candidate gene-based approach using case-control association settings. Here, we leverage a hypothesis-free whole genome sequencing in two two unrelated families (Family A and B) each with twins with a history of recurrent ACL ruptures acquired playing rugby as their primary sport, aimed to elucidate biologically relevant function-altering variants and genetic modifiers in ACL rupture.

Inhibition of SARS-CoV-2 replication in the lung with siRNA/VIPER polyplexes.

SARS-CoV-2 has been the cause of a global pandemic since 2019 and remains a medical urgency. siRNA-based therapies are a promising strategy to fight viral infections. By targeting a specific region of the viral genome, siRNAs can efficiently downregulate viral replication and suppress viral infection.

How Droplets Move on Surfaces with Directional Chemical Heterogeneities.

The nature of adhesion of droplets to surfaces is a long pending scientific question. With the evolution of complex surfaces, quantification and prediction of these adhesion forces become intricate. Nevertheless, understanding these forces is highly relevant for explaining liquid transport in nature and establishing design guidelines for manmade interfaces.

Light driven diffusioosmotic repulsion and attraction of colloidal particles.

In this paper, we introduce the phenomenon of light driven diffusioosmotic long-range attraction and repulsion of porous particles under irradiation with UV light. The change in the inter-particle interaction potential is governed by flow patterns generated around single colloids and results in reversible aggregation or separation of the mesoporous silica particles that are trapped at a solid surface. The range of the interaction potential extends to several times the diameter of the particle and can be adjusted by varying the light intensity.

Spatiotemporal Intermittency in Pulsatile Pipe Flow.

Despite its importance in cardiovascular diseases and engineering applications, turbulence in pulsatile pipe flow remains little comprehended. Important advances have been made in the recent years in understanding the transition to turbulence in such flows, but the question remains of how turbulence behaves once triggered. In this paper, we explore the spatiotemporal intermittency of turbulence in pulsatile pipe flows at fixed Reynolds and Womersley numbers (Re=2400, Wo=8) and different pulsation amplitudes.

Which factors are associated with bone marrow oedema suspicious of axial spondyloarthritis as detected by MRI in the sacroiliac joints and the spine in the general population?

Objective: Identify factors associated with presence and extension of spinal and sacroiliac joints (SIJ)-MRI lesions suggestive of axial spondyloarthritis (axSpA) in a population-based cohort (Study of Health in Pomerania) aged <45 years.

Methods: Spinal (sagittal T1/T2) and SIJ (semicoronal STIR sequences) MRIs were evaluated by two trained blinded readers. The presence (yes/no) and extension (Berlin MRI Score) of bone marrow oedema (BME) were captured.

Adsorption of Photoresponsive Surfactants at Solid-Liquid Interfaces.

We report on the adsorption kinetics of azobenzene-containing surfactants on solid surfaces of different hydrophobicity. The understanding of this processes is of great importance for many interfacial phenomena that can be actuated and triggered by light, since the surfactant molecules contain a photoresponsive azobenzene group in their hydrophobic tail. Three surfactant types are studied, differing in the spacer connecting the headgroup and the azobenzene unit by between 6 and 10 CH groups.

Nanoparticle-Mediated Gene Silencing for Sensitization of Lung Cancer to Cisplatin Therapy.

Platinum-based chemotherapy remains a mainstay treatment for the management of advanced non-small cell lung cancer. A key cellular factor that contributes to sensitivity to platinums is the 5'-3' structure-specific endonuclease excision repair cross-complementation group 1 (ERCC1)/ xeroderma pigmentosum group F (XPF). ERCC1/XPF is critical for the repair of platinum-induced DNA damage and has been the subject of intense research efforts to identify small molecule inhibitors of its nuclease activity for the purpose of enhancing patient response to platinum-based chemotherapy.

Extremely Long-Range Light-Driven Repulsion of Porous Microparticles.

The repulsive surface forces, such as electrostatic or steric, acting between particles explain why they remain well separated in aqueous electrolyte solutions and are responsible for the stability of colloidal dispersions. However, the effective range of these interactions is always well below hundreds of nanometers and typically can be controlled by advanced manipulations such as tuning the electrolyte concentration or modifying the particle surface or, in some more specific cases, via subjecting the suspension to an external electric or magnetic field. Here we employ solutions with small additives of a photosensitive ionic surfactant to investigate if a repulsive interaction of microsized particles sedimented at the solid surface can be remotely controlled simply by illuminating it with an appropriate wavelength.

Light driven guided and self-organized motion of mesoporous colloidal particles.

We report on guided and self-organized motion of ensembles of mesoporous colloidal particles that can undergo dynamic aggregation or separation upon exposure to light. The forces on particles involve the phenomenon of light-driven diffusioosmosis (LDDO) and are hydrodynamic in nature. They can be made to act passively on the ensemble as a whole but also used to establish a mutual interaction between particles.

Inflammatory stays inflammatory: a subgroup of systemic sclerosis characterized by high morbidity and inflammatory resistance to cyclophosphamide.

Background/purpose: Elevated levels of C-reactive protein (CRP) in systemic sclerosis (SSc) have been linked to early inflammatory stages of the disease. This study has been designed to investigate CRP levels longitudinally in a cohort of SSc patients and to correlate these findings with comorbidities and disease characteristics.

Methods: In this retrospective study, patients with SSc treated at the outpatient clinic of the Department of Rheumatology and Clinical Immunology, University Medical Center Freiburg, were analyzed.

Frequency of MRI changes suggestive of axial spondyloarthritis in the axial skeleton in a large population-based cohort of individuals aged <45 years.

Objective: To investigate the frequency of bone marrow oedema (BME) and fatty lesions (FL) suggestive of axial spondyloarthritis (axSpA) on MRI of the spine and sacroiliac joints (SIJ) in a general population sample.

Methods: As part of a community-based cohort project (Study of Health in Pomerania), volunteers underwent spinal (sagittal T1/T2) and SIJ (semicoronal short tau inversion recovery) MRI examinations. Two calibrated readers evaluated the images to detect BME in SIJ and vertebral corners (VC) and FL in VC suggestive of axSpA using Assessment of SpondyloArthritis international Society definitions.

Characterization of spray dried powders with nucleic acid-containing PEI nanoparticles.

Localized aerosol delivery of gene therapies is a promising treatment of severe pulmonary diseases including lung cancer, cystic fibrosis, COPD and asthma. The administration of drugs by inhalation features multiple benefits including an enhanced patient acceptability and compliance. The application of a spray dried powder formulation has advantages over solutions due to their increased stability and shelf life.

Microfluidic Assembly of siRNA-Loaded Micelleplexes for Tumor Targeting in an Orthotopic Model of Ovarian Cancer.

The use of cationic polymers to interact with negatively charged siRNA via charge complexation to form polyelectrolyte complexes has been widely studied ever since the 1998 report on RNA interference. These polyelectrolyte complex formulations aim to overcome the many pitfalls associated with the use of RNA interference as a potential cancer therapy. The triblock copolymer polyethylenimine-polycaprolactone-polyethylene glycol (PEI-PCL-PEG) contains the cation PEI and has been shown to be an efficient carrier capable of complexing with nucleic acids for gene delivery.

Baboon (Papio ursinus) single fibre contractile properties are similar to that of trained humans.

This study investigated the contractile properties of single skeletal muscle fibres from the Vastus lateralis (VL) of two male adult chachma baboons (Papio ursinus) and compared it to that from five male human cyclists. Species comparisons are observational and statistical analyses were not performed due to the low sample size. The histological analyses revealed that the baboon muscles contained more type II fibres than their human counterparts.

Evaluating the Regulation of Cytokine Levels After siRNA Treatment in Antigen-Specific Target Cell Populations via Intracellular Staining.

RNA interference (RNAi) offers a promising base for therapeutic knockdown of clinically relevant genes. Local delivery routes as well as targeted delivery to specific cell populations have been shown to circumvent several hurdles of successful siRNA delivery in vivo. To evaluate and quantify the treatment effect in a precise way, next to measuring the downregulation on gene and protein levels, it is equally essential to investigate the influence on downstream factors such as generated cytokines.

In vitro and in vivo delivery of siRNA via VIPER polymer system to lung cells.

The block copolymer VIPER (virus-inspired polymer for endosomal release) has been reported to be a promising novel delivery system of DNA plasmids both in vitro and in vivo. VIPER is comprised of a polycation segment for condensation of nucleic acids as well as a pH-sensitive segment that exposes the membrane lytic peptide melittin in acidic environments to facilitate endosomal escape. The objective of this study was to investigate VIPER/siRNA polyplex characteristics, and compare their in vitro and in vivo performance with commercially available transfection reagents and a control version of VIPER lacking melittin.

PARP-1/PAR Activity in Cultured Human Lens Epithelial Cells Exposed to Two Levels of UVB Light.

This study investigated poly(ADP-ribose) polymerase-1 (PARP-1) activation in cultured human lens epithelial cells exposed to two levels of UVB light (312 nm peak wavelength), 0.014 and 0.14 J cm ("low" and "high" dose, respectively).

Revisiting the value of competition assays in folate receptor-mediated drug delivery.

Polymeric nanoparticles have been studied for gene and drug delivery. These nanoparticles can be modified to utilize a targeted delivery approach to selectively deliver their payload to specific cells, while avoiding unwanted delivery to healthy cells. One commonly over-expressed receptor which can be targeted by ligand-conjugated nanoparticles is the folate receptor alpha (FRα).