PGC-based cryobanking, regeneration through germline chimera mating, and CRISPR/Cas9-mediated TYRP1 modification in indigenous Chinese chickens.

Primordial germ cells (PGCs) are vital for producing sperm and eggs and are crucial for conserving chicken germplasm and creating genetically modified chickens. However, efforts to use PGCs for preserving native chicken germplasm and genetic modification via CRISPR/Cas9 are limited. Here we show that we established 289 PGC lines from eight Chinese chicken populations with an 81.

Insights into the genetic diversity of indigenous goats and their conservation priorities.

Objective: An experiment was conducted to evaluate genetic diversity of 26 Chinese indigenous goats by 30 microsatellite markers, and then to define conservation priorities to set up the protection programs according to the weight given to within- and between-breed genetic diversity.

Methods: Twenty-six representative populations of Chinese indigenous goats, 1351 total, were sampled from different geographic regions of China. Within-breed genetic diversity and marker polymorphism were estimated calculating the mean number of alleles (MNA), observed heterozygosities (HO), expected heterozygosities (He), fixation index (FIS), effective number of alleles (NE) and allelic richness (Rt).

[Genetic diversity of seven miniature pig breeds (strains) analyzed by using microsatellite markers].

Twenty-seven microsatellite markers recommended by the Food and Agriculture Organization (FAO) and the International Society for Animal Genetics (ISAG) were used to analyze the genetic diversities of 7 Chinese native miniature pig breeds/strains (Jiuyang Xiang Pig, Jianbai Xiang Pig, Congjiang Xiang Pig, Huanjiang Xiang Pig, Black Xiang Pig, Wuzhishan Pig, and Diannan Small Ear Pig) and 3 western pig breeds (Duroc, Large White, and Landrace). The results indicate that there exists, in general, a high degree of genetic variability within the 7 miniature pig breeds. However, the genetic variability in Jiuyang Xiang Pig, Jianbai Xiang Pig, Huanjiang Xiang Pig, and Congjiang Xiang Pig (average PICs being 0.

Genetic diversity of microsatellite loci in fifty-six Chinese native pig breeds.

The genetic diversity of fifty-six indigenous pig breeds in China, and three introduced pig breeds (Duroc, Landrace and Large White) was surveyed using twenty-seven microsatellites recommended by the International Society for Animal Genetics (IS-AG) and Food and Agriculture Organization (FAO). By means of the allele frequencies, mean heterozygosity, effective number of alleles, estimator of gene differentiation, polymorphism information content, genetic distance and dendrogram analysis, the genetic variability and population structure of native pig breeds were estimated. Genetic variabilities within native pig breeds are as follows: Effective number of alleles vary from 2.