Infectious hematopoietic necrosis virus (IHNV) poses significant risks to salmonid fish, affecting both wild populations and aquaculture, making effective monitoring essential.
A new detection technology using reverse transcription and recombinase polymerase amplification (RT-RPA) combined with CRISPR/Cas12a has been developed to identify the IHNV N gene in a rapid two-step process.
This method can deliver results in under an hour with high sensitivity and specificity, allowing easy visual interpretation of results, which could greatly enhance IHNV infection control efforts.