Atrial natriuretic peptide protects vertebral endplate chondrocytes against HO‑induced apoptosis and oxidative stress through activation of the Nrf2/HO‑1 signaling pathway.

The present study aimed to investigate the effect of atrial natriuretic peptide (ANP) on cell apoptosis and oxidative stress in HO‑induced vertebral endplate chondrocytes (EPCs), and to assess the associated mechanisms involved. Cell viability and apoptosis were evaluated using the Cell Counting Kit‑8 method and TUNEL assay, respectively. In addition, the scavenging capability was detected using various enzymatic assays, and the quantity of nitric oxide (NO) and malondialdehyde (MDA), and activity of superoxide dismutase (SOD) were assessed.

Experimental study on the repair of ureteral functional regeneration with highly bioactive extracellular matrix stent.

Background: The research of extracellular matrix stent (ECM) has made some progress in the repair of urethra and bladder defects.

Objectives: To observe the effects of highly bioactive ECM scaffold on the regeneration and repair of defects in long-segment ureteral replacement.

Material And Methods: An animal model of long-segment ureteral defect was established and four-layer tubular highly bioactive ECM materials were prepared.