A highly sensitive and selective artificial nanochannel for in situ detection of hydroxyl radicals in single living cell.

As the most aggressive reactive oxygen species (ROS), hydroxyl radical (•OH) can directly modulate the biological ion channel and interfere with the progression of diseases. Inspired by biological •OH-activated ion channel, we reported a novel •OH-regulated glass nanopore functionalized with protoporphyrin Ⅸ (PP) film. This system showed outstanding •OH selective response owing to the ultra-fast reaction between •OH and thiol derivatives.