SIRT5 safeguards against primate skeletal muscle ageing via desuccinylation of TBK1.

Ageing-induced skeletal muscle deterioration contributes to sarcopenia and frailty, adversely impacting the quality of life in the elderly. However, the molecular mechanisms behind primate skeletal muscle ageing remain largely unexplored. Here, we show that SIRT5 expression is reduced in aged primate skeletal muscles from both genders.

Eupatilin mitigates ICH-induced brain injury via SOX2/SLC7A11 regulation of ferroptosis.

Intracerebral hemorrhage (ICH) is an acute and potentially fatal brain disease that causes severe brain damage, resulting in hemiplegia, speech disorders, and even coma or death. Recently, oxidative stress and ferroptosis are claimed involved in the pathogenesis of ICH brain injury. Eupatilin (EUP) is a flavonoid component with promising anti-inflammatory and anti-oxidative stress properties.

[LINC00837/miR-671-5p/SERPINE2 functional axis promotes pathological processes of fibroblast-like synovial cells in rheumatoid arthritis].

Objectives: To investigate the regulatory effect of LINC00837/miR-671-5p/SERPINE2 functional axis on pathological processes of fibroblast-like synovial cells (FLS) in rheumatoid arthritis (RA).

Methods: RA-FLS were transfected with a LINC00837 overexpression plasmid (pcDNA3.1-LINC00837), a LINC00837 interference plasmid (siRNA-LINC00837), or their respective negative control plasmids (pcDNA3.

Predicting the efficacy of neoadjuvant chemotherapy in breast cancer patients based on ultrasound longitudinal temporal depth network fusion model.

Objective: The aim of this study was to develop and validate a deep learning radiomics (DLR) model based on longitudinal ultrasound data and clinical features to predict pathologic complete response (pCR) after neoadjuvant chemotherapy (NAC) in breast cancer patients.

Methods: Between January 2018 and June 2023, 312 patients with histologically confirmed breast cancer were enrolled and randomly assigned to a training cohort (n = 219) and a test cohort (n = 93) in a 7:3 ratio. Next, pre-NAC and post-treatment 2-cycle ultrasound images were collected, and radiomics and deep learning features were extracted from NAC pre-treatment (Pre), post-treatment 2 cycle (Post), and Delta (pre-NAC-NAC 2 cycle) images.

Erratum: A single-cell transcriptomic atlas of exercise-induced anti-inflammatory and geroprotective effects across the body.

[This corrects the article DOI: 10.1016/j.xinn.

[ Polysaccharide Improves Reproductive Injury in Female Rats Exposed to High-Altitude Hypoxic Environment: Investigation of the Mechanisms Involved].

Objective: To investigate the protective effect of polysaccharide (LBP) on reproductive system damage induced by exposure to high-altitude hypoxic environment in female rats, and to explore the mechanisms involved.

Methods: After undergoing physiological synchronization, 30 female Wistar rats were randomly and evenly assigned to 3 groups, including a plain control (C) group, a high-altitude hypoxia (H) group, and a high-altitude hypoxia + LBP (H-LBP) group. The C group was placed in a region at an altitude of 1500 m above sea level (with an oxygen volume fraction of 18.

Fengshi gutong capsules attenuates CIA-induced RA bone destruction in rats by targeting TNF-α inhibition: Integration and experimental validation of network pharmacology and proteomics.

Ethnopharmacological Relevance: Fengshi Gutong Capsule (FSGT) is a proprietary Chinese medicine with established clinical efficacy in Rheumatoid arthritis (RA); however, its underlying mechanisms remain unclear.

Aim: This study aims to elucidate the mechanisms by which FSGT alleviates RA.

Materials And Methods: A collagen-induced arthritis (CIA) rat model was employed to assess the therapeutic effects of FSGT in RA.

Biochar-amended constructed wetlands enhance sulfadiazine removal and reduce resistance genes accumulation in treatment of mariculture wastewater.

With the rapid development of mariculture, an increasing amount of antibiotics are being discharged into the marine environment. Effectively removing antibiotics and antibiotic resistance genes (ARGs) in mariculture wastewater with a relatively high salinity and low C/N presents challenges. Biochar-amended constructed wetlands (CWs) can effectively remove antibiotics, However, few studies have compared the impacts of biochar-amended CWs pyrolyzed at different temperatures on the treatment of mariculture wastewater.

Climate change and air pollution can amplify vulnerability of glucose metabolism: The mediating effects of biological aging.

Climate change and air pollution pose significant global health threats, including impacts on diabetes risk; however, their long-term effects on insulin resistance (IR), a key determinant in diabetes pathophysiology, remain unclear. This study investigated whether exposure to heatwaves, temperature fluctuations, and warm-season ozone (O) contributes to or exacerbates IR and explored the potential mediating role of biological aging. The study enrolled 6901 participants and assessed both traditional and novel IR indicators: estimated glucose disposal rate (eGDR), triglyceride-glucose (TyG) index, triglyceride to high-density lipoprotein cholesterol ratio (TG/HDL-c), metabolic score for IR (METS-IR), TyG-body mass index (TyG-BMI), TyG-waist circumference (TyG-WC), waist-to-height ratio (WHtR), TyG-WHtR, and lipid accumulation product (LAP).

Population-level analyses identify host and environmental variables influencing the vaginal microbiome.

The vaginal microbiome is critical for the reproductive health of women, yet the differential impacts exerted by the host and by ambient environmental variables on the vaginal microbiome remain largely unknown. Here, we conducted a comprehensive cross-sectional study of the relationships between the vaginal microbiome and 81 matched host and environmental variables across 6755 Chinese women. By 16S rRNA sequencing, we identified four core vaginal microbiota with a prevalence of over 90% and a total median abundance of 98.

Discovery of structurally intriguing diterpenoids as anti-neuroinflammatory agents from mangrove plant Excoecaria agallocha L. via inhibiting macrophage polarization and inflammasome.

Emerging evidence indicates the inhibition of neuroinflammation is an important and promising strategy for managing Alzheimer's disease. Anti-neuroinflammatory agents are potential drug leads. In this work, seventeen diterpenoids (1-17) including ten previously undescribed ones, named excoaglolides A-J (1-10) were isolated from the stems and twigs of the mangrove plant, Excoecaria agallocha L.

Efficacy and safety of lateral lumbar interbody fusion for lumbar degenerative diseases with or without auxiliary posterior fixation: a meta-analysis and systematic review.

Background: There is no conclusive evidence from evidence-based medicine that clarifies whether the efficacy and safety of lateral lumbar interbody fusion (LLIF) differ significantly with or without auxiliary posterior fixation. This study embarks on a comprehensive comparative meta-analysis, delving into both domestic and international research landscapes, to scrutinize the efficacy of stand-alone LLIF versus LLIF coupled with auxiliary posterior fixation in the treatment of degenerative lumbar diseases.

Methods: In this meta-analysis, we searched Pubmed, Embase, and Cochrane databases from inception to December 2023.

The Sirt1/FOXO signal pathway involves in regulating osteomyelitis progression via modulating mitochondrial dysfunctions and osteogenic differentiation.

The Sirtuin-1 (Sirt1) gene has been reported to be closely associated with the progression of multiple diseases, but its role in regulating osteomyelitis (OM) pathogenesis has not been explored. The murine long bone-derived osteocyte-like MLO-Y4 cells and osteoblast-like MC3T3-E1 cells were exposed to Staphylococcal protein A (SpA) treatment to establish the in vitro OM models. The expression levels of Osteoblast-specific genes (OCN, OPN and RUNX2), osteoclastic genes (CTSK, MMP9 and ACP5) and the FOXO pathway-related proteins (FOXO1, p-FOXO1, FOXO3 and p-FOXO3) were detected by performing Real-Time qPCR and Western Blot analysis.

Identification of serum phosphatidylinositol glycan anchor biosynthesis class U protein as diagnostic biomarker for breast cancer.

Background: Finding markers of breast cancer that are strong specificity and high. Sensitivity is important. The expression of phosphatidylinositol U（PIGU） protein is increased in a variety of tumor cells, but the role of its secreted fragment in breast cancer is lacking.

Chronic stress-induced cholesterol metabolism abnormalities promote ESCC tumorigenesis and predict neoadjuvant therapy response.

Recent studies have demonstrated that chronic stress can enhance the development of multiple human diseases, including cancer. However, the role of chronic stress in esophageal carcinogenesis and its underlying molecular mechanisms remain unclear. This study uncovered that dysregulated cholesterol metabolism significantly promotes esophageal carcinogenesis under chronic stress conditions.

Impact of microgeography on communication dynamics in a healthcare environment.

Background: For growing healthcare organisations, anchored resources-assets that are not easily movable-may complicate expansion and distort workflow patterns. We examine work patterns at a radiation oncology department of a major Canadian hospital. As this department doubled its size, healthcare providers remained bound to treatment planning rooms and radiation machines at the original site.

Desflurane attenuates renal ischemia-reperfusion injury by modulating ITGB1/CD9 and reducing oxidative stress in tubular epithelial cells.

Objective: Renal ischemia-reperfusion (I/R) injury triggers significant oxidative stress and inflammation, leading to tubular epithelial cell (TEC) damage. This study investigates the protective role of Desflurane (DFE) in renal I/R by modulating the ITGB1/CD9 signaling pathway and mitigating oxidative damage.

Methods: Single-cell RNA sequencing (scRNA-seq) and transcriptome analysis identified ITGB1 as a key regulatory gene in TECs during renal I/R.

External Validation of a 5-Factor Risk Model for Breast Cancer-Related Lymphedema.

Importance: Secondary lymphedema is a common, harmful side effect of breast cancer treatment. Robust risk models that are externally validated are needed to facilitate clinical translation. A published risk model used 5 accessible clinical factors to predict the development of breast cancer-related lymphedema; this model included a patient's mammographic breast density as a novel predictive factor.

Immune-modulating nanomedicines for enhanced drug delivery to non-small-cell lung cancer.

Immune-modulating peptides have shown potential as novel immune-stimulating agents which enhance the secretion of anticancer cytokines in vitro. However, fast clearance from blood hampers the ability of such peptides to accumulate in the tumour and results in limited therapeutic efficacy in animal studies. To address the fast blood clearance, this work reports the development and validation of a novel polymeric nanoparticle delivery system for the efficient localization of an immunomodulating peptide in the tumour microenvironment (TME).

Temperature influence on matric suction in unsaturated soils: experimental insights.

Temperature is a critical factor affecting the matric suction of unsaturated soils. This study employed a simple and time-saving method to investigate the influences of temperature and moisture content on the temperature dependence of matric suction in loess and sand soils. Based on experimental data, the fitting performance of three data-fitting models-Brooks-Corey, van Genuchten, and Fredlund-Xing -was evaluated at temperatures above 0 °C.

[Xinfeng Capsule alleviates RA-FLS-induced angiogenesis in HUVEC cells by inhibiting the lncRNA HOTAIR/PI3K/AKT pathway].

Objective To investigate the effect of serum containing Xinfeng capsule (XFC) on the angiogenesis of human umbilical vein endothelial cells (HUVEC) induced by rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) and its mechanism of action. Methods An in vitro co-culture model of RA-FLS and HUVEC was established. Serum containing XFC was prepared by oral gavage of SD rats.

Estimating the Risk of Women Anemia Associated with Ozone Exposure Across 123 Low- and Middle-Income Countries: A Multicenter Epidemiological Study.

Anemia in women of reproductive age (WRA) presents a pressing global public health issue, particularly in low- and middle-income countries (LMICs). Yet, the potential impact of ozone (O) exposure on anemia remains uncertain. The study included 1,467,887 eligible women from 83 surveys of 45 LMICs between 2004 to 2020.

p53 and Ki-67 combined with periodic acid-Schiff staining for the diagnosis of early stage esophageal squamous cell carcinoma lesions in biopsy specimens.

Background: Esophageal cancer is highly prevalent in China, predominantly represented by squamous cell carcinoma. This retrospective study sought to evaluate the diagnostic efficacy of four staining protocols in identifying early stage esophageal squamous cell carcinoma (ESCC).

Methods: A consecutive series of ninety biopsy samples of esophageal mucosa, collected retrospectively from March 2016 to December 2019, were obtained at Beijing Chao-Yang Hospital, a tertiary care facility in Beijing, China.

AH-6809 mediated regulation of lung adenocarcinoma metastasis through NLRP7 and prognostic analysis of key metastasis-related genes.

Introduction: Lung adenocarcinoma (LUAD) has become one of the leading causes of cancer-related deaths globally, with metastasis representing the most lethal stage of the disease. Despite significant advances in diagnostic and therapeutic strategies for LUAD, the mechanisms enabling cancer cells to breach the blood-brain barrier remain poorly understood. While genomic profiling has shed light on the nature of primary tumors, the genetic drivers and clinical relevance of LUAD metastasis are still largely unexplored.

Structural Characterization and Hypoglycemic Activity of a Glycoprotein Extracted from .

Glycoproteins are special proteins and important nutrients for hypoglycemic activity. However, the structure of glycoprotein (AAG) and the stability of its hypoglycemic activity during simulated digestion (including saliva, gastral and intestine digestion) in vitro are still unknown. In this study, AAG-3 was isolated from .