[Research progress of mitochondrial fission and fusion related proteins].

Mitochondria are an essential component of multicellular life and play important roles in the health of the cells and the body. Mitochondria can produce energy by oxidative phosphorylation, mediate calcium and reactive oxygen signal transduction, and regulate cell apoptosis. Recent studies indicate that mitochondria continually change their shapes and distribution by fission and fusion, which are collectively termed mitochondrial dynamics.

Diosgenin Glucoside Protects against Spinal Cord Injury by Regulating Autophagy and Alleviating Apoptosis.

Spinal cord injury (SCI) is a severe traumatic lesion of central nervous system (CNS) with only a limited number of restorative therapeutic options. Diosgenin glucoside (DG), a major bioactive ingredient of Max., possesses neuroprotective effects through its antioxidant and anti-apoptotic functions.

Identification of p90 Ribosomal S6 Kinase 2 as a Novel Host Protein in HBx Augmenting HBV Replication by iTRAQ-Based Quantitative Comparative Proteomics.

Purpose: The aim of this study was to screen for novel host proteins that play a role in HBx augmenting Hepatitis B virus (HBV) replication.

Experimental Design: Three HepG2 cell lines stably harboring different functional domains of HBx (HBx, HBx-Cm6, and HBx-Cm16) were cultured. ITRAQ technology integrated with LC-MS/MS analysis was applied to identify the proteome differences among these three cell lines.

Biological characteristics of the A1762T/G1764A mutant strain of hepatitis B virus in vivo.

The double nucleotide, A1762T and G1764A exchange (TA mutation), in the hepatitis B virus (HBV) genome basal core promoter (BCP) region is a common viral mutation in patients with chronic HBV infection. This mutation is located in the binding site of hepatocyte nuclear factor 4 (HNF4), and a number of liver‑enriched transcription factors are involved in the regulation of HBV transcription and replication. The aim of the present study was to investigate the biological characteristics of the HBV strain with this mutation, and the effect of HNF4 inhibition on the replication of this strain in vivo.

Role and functional domain of hepatitis B virus X protein in regulating HBV transcription and replication in vitro and in vivo.

The role of hepatitis B virus (HBV) X protein (HBx) in the regulation of HBV replication remains controversial. In the present study, the role of HBx in regulating HBV replication was initially investigated in both HepG2 and Huh7 in vitro cell lines with a transient transfection system. Next, the regions of HBx responsible for transcriptional transactivation and promotion of HBV replication were mapped in an HBV replication mouse model by in vivo transfection of a series of HBx expression plasmids.

Biological characteristics of the rtA181T/sW172* mutant strain of Hepatitis B virus in animal model.

Background: The effects of Hepatitis B virus (HBV) rtA181T/sW172* mutation on viral replication and pathogenicity was concerned recently. This study aimed to investigate the biological characteristics of rtA181T/sW172* mutant strain of HBV in animal model.

Methods: The rtA181T/sW172* mutant plasmid was constructed using the pHBV4.

Study of the expression levels of Hepatocyte nuclear factor 4 alpha and 3 beta in patients with different outcome of HBV infection.

Hepatocyte nuclear factors 4 alpha (HNF4α) and 3 beta (HNF3β) are members of a group of liver-enriched transcription factors (LETFs) that play important roles in regulating the replication of hepatitis B virus (HBV) and liver inflammation. However, the relationship of the level of HNF4α and HNF3β with the severity of HBV-infected liver diseases is unclear. In this study, liver tissue samples from different types of HBV patients were collected, and HNF4α and HNF3β expression were detected by immunohistochemistry.

[Forensic pathological analysis on 23 cases of fatal pulmonary thromboembolism].

Objective: To analyze the forensic pathological characteristics of sudden death caused by pulmonary thromboembolism and the chronological transformation of thrombus and explore the assessment method of the causal relationship between previous trauma and the following fatal PTE episode.

Methods: All the 23 cases reviewed here were collected from our institute files from the year of 1998 to 2008.

Results: Trauma, surgery and braking etc.

Establishment of cell lines using a doxycycline-inducible gene expression system to regulate expression of hepatitis B virus X protein.

The hepatitis B virus (HBV) X gene plays an important role in HBV-associated pathogenesis, especially hepatocarcinogenesis. Establishment of a stable and regulable HBx expression system will allow study of the function of this gene. Here, we describe the development of a doxycycline-inducible recombinant plasmid (pBPSTR3-FlagX) with the full-length HBV X gene and all components of the tetracycline-on ("Tet-on") gene expression system.

[Analysis of death caused by postpartum hemorrhage].

Objective: To analyze the pathological characteristics and the death reasons due to postpartum hemorrhage, and to help to deal with the obstetrical medical tangles.

Methods: Thirty-two cases of death caused by postpartum hemorrhage encountered in our department since 1995 had been collected and retrospectively analyzed.

Results: Death caused by postpartum hemorrhage could be divided into single factor and multi-factor, with 81.

[Effects of ginsenoside Rg1 on expression of insulin-like growth factor-1 in brain of rats with brain contusion].

Objective: To study the effects of ginsenoside Rg1 on the expression of insulin-like growth factor-1 (IGF-1) in the brain of rats after the experimental brain contusion.

Methods: A total of twenty-six Wistar rats were randomly divided into normal control group (n=2), untreated group (n=8) and ginsenoside Rg1 group (n=16). Brain injuries were induced in rats by a mechanical striking device.

[Expression of myocardial collagen I in early acute myocardial ischemic of human].

Objective: To estimate the significance of myocardial collagen I in the early acute myocardial ischemia of human.

Methods: The myocardial paraffin block had normal group, early myocardial ischemia group and myocardial infarction group. The myocardial collagen I was observed with immunohistochemical staining and analyzed by half-quantity.

[Sex determination by studying head CT film].

Objective: To explore the methods for sex determination on head CT film.

Methods: To establish the sex determination equations by binary regression analysis of cranial skeletal indices obtained on head CT film.

Results: A single element equation by applying the index of the width of ansa capitis and a multi-element equation by applying multitude indices were established.

[Comparative study for the methods on measuring intracranial hemorrhage volume].

Objective: To explore a method of measuring intracranial hemorrhage to be applied in clinical forensic science.

Methods: The accurace of the methods of Tada formula, Stereology and Software boundary to measure intracranial hemorrhage and their practicability were compared.

Results: The measured results of Stereology and Software boundary are precise, but the irregularity haematoma volume measured by the method of Tada formula has a high error value.

[On the role of liver-enriched transcription factors in regulating HBV transcription and replication].

Objective: To investigate the effects of various liver-enriched transcription factors in regulating HBV transcription and replication, and to explore their potential roles in HBV hepatotropism.

Methods: The replication-competent HBV recombinant plasmid pHBV4.1 plus different liver-enriched transcription factor (HNF1, HNF3, HNF4, HNF6, C/EBP and RXRa/PPARa) expression plasmids were co-transfected into nonhepatic cell lines (NIH3T3, HeLa, 293T, SW1353, CV-1 and COS1).