Ocular findings in syndromic gingival fibromatosis: a case study and electronic microscopic investigation of lens.

We report a case of syndromic gingival fibromatosis with notable ocular lesions, bilateral congenital cataracts, esotropia, and high myopia of a 21-year-old male patient from China. The patient was diagnosed with gingival fibromatosis based on his massive gingival overgrowth and histological findings that were consistent with gingival fibromatosis through a gingival biopsy. Lens opacity features were presented and phacoemulsificaion with intraocular lens(IOL) implantation was performed to manage the cataracts in both eyes.

Gingival fibromatosis with multiple unusual findings: report of a rare case.

Gingival fibromatosis is a rare disease, especially its syndromic form. Here, we review the literatures on gingival fibromatosis and briefly summarize some characters on clinical, etiological, genetic and histopathological aspects. We also present a rare case of gingival fibromatosis with multiple unusual findings in a 21-year-old man.

[Application of computer aided design-computer aided manufacture technique in mandible defect reconstruction with individual titanium prosthesis].

Objective: To investigate the application of computer aided design-computer aided manufacture (CAD-CAM) technique in the reconstruction of mandible defect with individual titanium prosthesis.

Methods: Six patients with large mandibular ramus and angle tumor were spiral CT scanned preoperatively, and the CAD-CAM was used to design and make individual titanium prosthesis for reconstructing the mandibular defects after resection of the tumor. The prosthesis were assembled during operation.

[Application of three dimensional model in evaluating the effect of artificial bone implantation of hard cleft palate on the development of maxilla].

Objective: To observe the effect of artificial bone implantation of hard cleft palate on the development of maxilla.

Methods: From January 1997 to December 1999, 40 patients with hard cleft palate were randomly divided into two groups: control group and implantation group (n = 20 each). The patients in the implantation group received an implantation of compound artificial bone of HA-Bone cement.

[Clinical application of radiotherapy for the treatment of giant vascular malformations in oral and maxillofacial region].

Objective: To discuss the clinical use of radiotherapy in the treatment of giant vascular malformation.

Methods: Six patients with giant vascular malformation in oral and maxillofacial region were treated by three dimensional radiation therapy in Department of Stomatology, The Second Affiliated Hospital, School of Medicne, Zhejiang University in the last ten years and the cilinical data were reviewed. The treatment results were evaluated by clinical examination and radiology.

[Computer-aided design in double-step distraction osteogenesis of unilateral mandibular segmental defects reconstruction].

Objective: To investigate the feasibility of using computer-aided design (CAD) in double-step distraction osteogenesis in the reconstruction of mandibular segmental defects after tumor resection.

Methods: Eight cases of unilateral mandibular segmental defects were reconstructed using distraction osteogenesis secondary to oncologic surgery, with the help of CT and CAD system. The mandibular body was lengthened first, and then the residual defect of mandibular ramus was restored using a distraction device.

[Cross-talk between Notch1 and epidermal growth factor receptor signalling in regulating cell proliferation of human tongue squamous carcinoma cells].

Objective: To investigate the cross-talk between Notch1 and epidermal growth factor receptor (EGFR) signaling in regulating the cellular proliferation of human tongue squamous cell carcinoma (SCC).

Methods: Human tongue SCC cell line Tca8113 cells was transiently transfected with the vector encoding exogenous intracellular fragment of Notch1 and the vector encoding the specific short hairpin RNA (shRNA) targeting EGFR respectively and were treated by AG1478, an inhibitor of receptor tyrosine kinases, for elucidating the effects of constitutive activation, EGFR gene silencing and blocking EGFR signaling upon cellular proliferation and expression of Notch1 and EGFR. The mRNA and protein levels of Notch1 and EGFR were detected by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blot, respectively.

[Application of saliva protein fingerprints in the diagnosis of oral squamous cell cancer by surface enhanced laser desorption ionization time of flight mass].

Objective: To screen proteomic markers of oral precancerous lesion, local and metastatic squamous cell cancer from human saliva.

Methods: Seventeen patients with oral squamous cell carcinoma, six cases with leukoplakia, seven cases with local metastasis and 15 healthy subjects were selected. All patients were diagnosed of T(1)N(0)M(0) and did not accept any preoperative treatment.

[Effects of epidermal growth factor receptor gene silencing mediated by short hairpin RNA on proliferation and apoptosis of human tongue carcinoma cells].

Objective: To investigate the effects of epidermal growth factor receptor (EGFR) gene silencing mediated by short hairpin RNA (shRNA) on proliferation and apoptosis of human tongue carcinoma cells.

Methods: shRNA eukaryotic expression vector targeting the specific sequence of human EGFR gene was constructed and termed shEGFR. The control vector targeting the unrelated sequence was also constructed and termed shNC.

Downregulation of Notch1 and its potential correlation with epidermal growth factor receptor signalling in tongue squamous cell carcinoma.

We investigated the expression of Notch1 in human oral squamous cell carcinoma (SCC) and explored its potential correlation with epidermal growth factor receptor (EGFR) signalling in oral SCC. Paraffin sections of primary SCC of the tongue and normal mucosa were screened immunohistochemically for Notch1 and EGFR proteins. Human SCC of the tongue Tca8113 cells were treated with AG1478 to block EGFR signalling, and were transfected with the vector that encodes the specific short hairpin RNA (shRNA) that targets EGFR.

[Soft tissue profile changes in micrognathia after distraction osteogenesis].

Objective: To evaluate the effect of distraction osteogenesis for severe micrognathia by comparing the pre- and post-operative profile and mentolabial relationship.

Methods: 16 cases underwent temporal-mandibular joint plasty and temporal fasciomuscular flap transfer. The mandibular distraction began at the 5th postoperative day at a rate of 0.

[Double-step distraction osteogenesis in the reconstruction of unilateral mandibular segmental defects after tumor resection].

Objective: To investigate the application of double-step distraction osteogenesis in the reconstruction of mandibular segmental defects after tumor resection.

Methods: From January 2002 to December 2006, six cases of post-tumor unilateral mandibular segmental defects were reconstructed using distraction osteogenesis. The mandibular body was lengthened first, following by mandibular ramus distraction.

[Application of computer aided design/computer aided manufactured techniques in mandible defect reconstruction].

Objective: To investigate the application of CAD/CAM technique in reconstruction of mandible defect.

Methods: Before surgery, three-dimensional computed tomography was performed in 23 patients with mandible lesions, then the CT data were transformed into a readable format to produce facsimile models by using rapid prototyping (RP) techniques. When individual mandibular models were produced, evaluation and surgical simulation were performed on the model, which included measuring range of mandible lesions, prefabrication of mandibular reconstructive titanium plates or distractors, ascertaining precise position of transport disk, and shaping the free vascularized fibula, etc.

[Application of CAD/CAM techniques in mandible large-scale defect and reconstruction with vascularized fibular bone graft].

Objective: To exploit computer-aided design and computer-aided manufactured (CAD/CAM) techniques and application in the reconstruction of mandible large-scale defect with vascularized fibular bone graft.

Methods: Before actually performing surgery, three-dimensional(3D) computed tomography(CT) was performed in 7 patients with mandibular large-scale defects, and 3D CT images were acquired by processing CT data. Then the CT data were transformed into a readable format and transferred to produce facsimile models by means of using rapid prototyping(RP) techniques.

Deep lingual arterial chemoembolization of tongue carcinoma with microcapsuled anticancer drug.

Objective: Microcapsule chemoembolism is a promising treatment of tumors. We describe a deep lingual arterial embolization of tongue carcinoma with microcapsuled carboplatinum.

Methods: Lingual artery cast specimens from cadavers were microscopically examined, and 78 patients with tongue cancer were recruited and treated with the deep lingual arterial embolization therapy.

[The application of three dimensional craniomaxillofacial model in the restoration of mandibular bone defects with vascularized fibular autograft].

Objective: To evaluate the effect of applying the three dimensional craniomaxillofacial model technique in the restoration of mandibular defects with vascularized fibular autograft.

Methods: Three dimensional craniomaxillofacial models of 24 patients with mandibular defects were achieved by CT scan and rapid prototype technique. Surgery simulation and individual prefabricated titanium board were designed on the models.

[The effect of artificial bone implantation in cleft palate on the development of maxilla].

Objective: To research the influence of palate bone implantation to the growth of maxilla in three dimensional direction.

Methods: 40 patients of congenital cleft palate type II with palate board damage were stochastically divide into the comparison group and bone planted group. In bone planted group, HA-Bone cement was used to repair palate tabular damage.

[Relative quantification of cytokeratin 19 transcription in oral squamous cell carcinoma tissues by fluorescent quantitive real-time RT-PCR].

Objective: To investigate the relative quantification of cytokeratin 19 transcription in oral squamous cell carcinoma tissues by fluorescent quantitive real-time reverse transcription-polymerase chain reaction ((RT-PCR).

Methods: CK19mRNA level was detected by fluorescent quantitive real-time RT-PCR in cancerous and para-cancerous tissues from 31 oral squamous cell carcinoma patients. According to the 2(-DeltaDeltaCt) equation, the relative quantification fold of CK19mRNA level was calculated in cancerous tissues compared with para-cancerous tissues.

Increased levels of CK19 mRNA in oral squamous cell carcinoma tissue detected by relative quantification with real-time polymerase chain reaction.

Oral squamous cell carcinoma (OSCC) is the most common malignant tumour in the oral and maxillofacial region and has a poor prognosis. Cytokeratin 19 (CK19) is a component of cytoskeleton protein. Previous studies have reported abnormal expression of CK19 protein in OSCC tissue.

[Langerhans' cell histiocytosis of the mandible in infant: report of one case].

Langerhans' cell histiocytosis of the mandible is a rare disease in infant. This article reported a case of Langerhans' cell histiocytosis of mandible in a 2-year old female child. The clinical manifestation, radiographic characteristics, pathologic diagnosis, differential diagnosis and treatment were discussed.