Publications by authors named "Fei-Lun Cui"
Article Synopsis
- - The study investigates the role of Methyltransferase-like 3 (METTL3) in cochlear hair cell damage caused by the antibiotic kanamycin, as its specific influence on ototoxicity was previously unclear.
- - Experiments showed that kanamycin treatment decreased METTL3 expression and led to the formation of stress granules (SGs) in both cochlear explants and HEI-OC1 cells, indicating a potential protective mechanism.
- - The research suggests that enhancing METTL3 activity can reduce reactive oxygen species (ROS) and apoptosis in hair cells, indicating its potential as a target for preventing damage from kanamycin exposure.
[Regulatory effect of MTA1 on the anoikis of human prostate cancer cells].
Zhonghua Nan Ke Xue
May 2011
Objective: To study the effects of MTA1 small interfering RNA (siRNA) on the anchorage-independent growth and anoikis of prostate cancer cell line PC-3.
Methods: After transfection of human prostate cancer PC-3 cells by MTA1 siRNA, we detected the expression of the MTA1 gene by real-time PCR and Western blot, the anchorage-independent growth of the cells by clone formation in soft agar, and their anoikis by DNA fragmentation assay and flow cytometry.
Results: Compared with the control group, MTA1 siRNA transfection significantly decreased the mRNA and protein levels of MTA1, inhibited the anchorage-independent growth of the PC-3 cells, and induced their anoikis, all in a dose- and time-dependent manner (r = 0.
[Expression of pituitary tumor transforming gene in human renal clear cell carcinoma and its significance].
Nan Fang Yi Ke Da Xue Xue Bao
July 2010
Objective: To explore the expression of pituitary tumor transforming gene (PTTG) in human renal clear cell carcinoma (RCCC) and its significance.
Methods: PTTG protein expression was detected by immunohistochemistry in 87 RCCC and 45 paired normal renal tissues.
Results: PTTG was expressed differentially between the normal renal and RCCC tissues.
Objective: To study the effect of laminarin sulphate (LAMS) on the expressions of PTEN and P27kip1 in androgen-independent prostate cancer PC-3 cells in vitro, and investigate the mechanism of its anti-tumor action.
Methods: The inhibitory effects of different concentrations of LAMS (0, 50, 100, 200 microg/ml) on androgen-independent prostate cancer PC-3 cells were detected by WST-8 assay. The morphology of PC-3 cells was observed under the fluorescence microscope, and the cell cycle and apoptosis were analyzed by flow cytometry.
Objective: To investigate the effects of adenovirus-mediated PTEN and P27 on the invasion of PC-3 in vitro and angiogenesis, along with their synergy in the treatment of prostate cancer.
Methods: Recombinant adenovirus vectors of the human tumor suppressor genes PTEN and P27 were constructed. The replication-incompetent recombinant adenovirus was packaged and propagated in HEK293 cells.