The N-terminal His-tag affects the triglyceride lipase activity of hormone-sensitive lipase in testis.

The sterility of hormone-sensitive lipase (HSL) knockout mice clearly shows the link between lipid metabolism and spermatogenesis. However, which substrate or product of this multifunctional lipase affects spermatogenesis is unclear. We found that an HSL protein with a His-tag at the N-terminus preserved the normal hydrolase activity of cholesteryl ester (CE) but the triglyceride lipase (TG) activity significantly decreased in vitro.

Association Between Nitrite and Nitrate Intake and Risk of Gastric Cancer: A Systematic Review and Meta-Analysis.

BACKGROUND Studies have shown inconsistent associations of nitrite and nitrate intake with the risk of gastric cancer or its associated mortality. We performed a meta-analysis of observational studies to evaluate the correlation of nitrite and nitrate intake with the risk of gastric cancer. MATERIAL AND METHODS We searched for studies reporting effect estimates and 95% confidence intervals (CIs) of gastric cancer in PubMed, EMBASE, and the Cochrane Library through November 2018.

[The development of genetics teaching in China in the last four decades and its future prospect].

Chinese genetics educators have carried out a comprehensive and systematic exploration and reform since 1978. With the guidance and help of the Genetics Society of China, they have made significant strides in the fields of genetics teaching system, publication of genetics textbooks, content of genetics teaching, workshop on genetics teaching, experimental teaching, application of advanced techniques, etc. These efforts have made remarkable achievements and promoted the vitality of genetics.

Negative regulation of RIG-I-mediated innate antiviral signaling by SEC14L1.

Retinoic acid-inducible gene I (RIG-I) is a key sensor for recognizing nucleic acids derived from RNA viruses and triggers beta interferon (IFN-β) production. Because of its important role in antiviral innate immunity, the activity of RIG-I must be tightly controlled. Here, we used yeast two-hybrid screening to identify a SEC14 family member, SEC14L1, as a RIG-I-associated negative regulator.

The distribution of genetic diversity of KIR genes in the Chinese Mongolian population.

Killer cell immunoglobulin-like receptors (KIRs) are expressed on natural killer cells and as such regulate their response against infection and malignancy. KIR genes are variable in gene content and type, which results in different KIR haplotypes, and can be used to discriminate individuals and populations from different regions or ethnic groups. In the present study, we represent the first report on the KIR gene frequency and content diversities of 14 KIR genes (KIR2DL1, 2DL2, 2DL3, 2DL4, 2DL5, 2DS1, 2DS2, 2DS3, 2DS4, 2DS5, 3DL1, 3DL2, 3DL3, 3DS1) and 2 pseudogenes (KIR3DP1 and 2DP1) in the Chinese Mongolian population.

[Application of case-based method in genetics and eugenics teaching].

Genetics and Eugenics is a cross-discipline between genetics and eugenics. It is a common curriculum in many Chinese universities. In order to increase the learning interest, we introduced case teaching method and got a better teaching effect.

Genetic variation of new 21 autosomal short tandem repeat loci in a Chinese Salar ethnic group.

In the present study, we reported the allele frequencies for new 21 autosomal short tandem repeat (STR) loci, including D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D19S433, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435 and D5S2500 loci. Forensic statistical parameters were estimated from a sample set of 120 unrelated healthy individuals from the Salar ethnic group in Xunhua Salar Autonomous County of Qinghai province, China. A total of 151 alleles were observed at 21 STR loci in the population, and their allele frequencies were in the range of 0.

[The exploration and implication of the concept map in genetics teaching.].

The concept map is a kind of chart used to represent and reveal the relationship among the knowledge structures. In genetics teaching, selectively using the concept map can help teachers to organize and review the course contents and also to detect students' academic achievement. It can also promote students' cognitive level, assist students to establish a system integrated knowledge hierarchy, etc.

[Exploration and practice of genetics teaching assisted by network technology platform].

More teaching techniques have been brought out gradually along with the development of new technologies. On the basis of those traditional teaching methods, a new platform has been set up by the network technology for teaching process. In genetics teaching, it is possible to use the network platform to guide student studying, promote student's learning interest and study independently by themselves.

[Peptide Nucleic Acids (PNA)--the explorer of gene mystery].

Peptide Nucleic Acids(PNA) are DNA analogues in which the naturally occurring nucleobases are attached via methylene carbonyl linkages to an achiral pseudopeptide backbone of N-(2-aminoethyl) glycine units. PNA can bind to both DNA and RNA targets in a sequence-specific manner. PNA provides a powerful tool to study the mechanism for gene replication and transcription as well as an innovative strategy to regulate target gene expression.

[On the research of histone methylation].

The types of histone methyltransferases, the relationship between methylation of Lysine 9 of H3 and the formation of heterochromatin, gene regulation in euchromatin, and that with DNA methylation, were mainly introduced. The interrelation between histone methylation and histone acetylation/phosphorylation was summarized. It is showed that histone methylation plays a very important role in maintaining the balance state of cell.

[The role of the carboxy-terminal domain of the RNA polymerase II on coupling mRNA transcription with processing].

The carboxy-terminal domain of the largest subunit of the RNA polymerase II (CTD) is comprised of multiple repeats of a conserved heptapeptide with the consensus sequence-Tyr-Ser-Pro-Thr-Ser-Pro-Ser-. In the past decade,much attention has been payed to this specific structure. Many experiments showed that the CTD played a pivotal role in the coupling mechanism that links mRNA transcription with processing by associating with the transcriptional and processing factors.