Coronectomy in Lower Third Molar Surgery: A Systematic Review and Meta-Analysis.

Purpose: Coronectomy is an alternative approach to the complete removal of an impacted mandibular third molar. The present study conducted a systematic review and meta-analysis to compare coronectomy with extraction concerning complications associated with the inferior alveolar nerve injury (IANI).

Methods: This review followed the principles of systematic reviews and meta-analyses.

[Effect of zoledronic acid on mandibular socket healing process of mice at an early stage].

Purpose: To investigate how bisphosphonates affect early process of socket healing in mice model.

Methods: Eighteen 8-9 weeks C57BL/6 male mice were randomly divided into control and experimental group. Bisphosphonate-related osteonecrosis of the jaw（BRONJ）model was conducted by intraperitoneal injection of zoledronate（ZOL） and extraction of lower left first molar in mice.

[Expression of hypoxia inducible factor-1alpha during mouse mandibular first molar eruption and its significance].

Purpose: To investigate the expression of hypoxia inducible factor-1alpha (HIF-1α) during the eruption of mouse lower first molar, and to explore the correlation between its expression and the activity of osteoclasts.

Methods: Mouse mandibles were dissected from mice at day 1.5 to 14.

[Mechanism of participation of osteocytes in the formation of osteoclasts under hypoxia].

Objective: To investigate the mechanism of the participation of osteocytes in the formation of osteoclasts under hypoxia.

Methods: The hypoxia culture system of osteocyte-like cell line MLO-Y4 was established by deferoxamine mesylate (DFO) in vitro. The proliferation of MLO-Y4 cells was examined by CCK-8 cell proliferation/toxicity assay.

HIF-1α facilitates osteocyte-mediated osteoclastogenesis by activating JAK2/STAT3 pathway in vitro.

Osteocytes, entrapped within the mineralized bone matrix, has been found to have numerous functions such as acting as an orchestrator of bone remodeling through regulation of both osteoclast and osteoblast activity and also functioning as an endocrine cell. Due to a specialized morphology and surrounding structure, osteocytes are more tolerant to hypoxia during osteoporosis, fracture, osteoarthritis, and orthodontic-orthognathic combination therapy. Hypoxia-inducible factor-1α (HIF-1α) is one of the master regulators of hypoxia reactions, playing an important role in bone modeling, remodeling, and homeostasis.

Role of CD133 cells in tongue squamous carcinomas: Characteristics of 'stemness' and .

The objective of the present study was to determine the 'stemness' characteristics of CD133 cells (harvested from the squamous cell tongue carcinoma Tca-8113 cell line) and to observe the tumourigenicity of the CD133 cells in the bodies of NOD/SCID mice. Single cells from the Tca-8113 cell line were observed for multiplication capacity . The suspending and pelletizing phenomena of Tca-8113 cells were also observed, and the expression of CD133 in squamous cell carcinoma of the tongue was measured.

Hypoxia-inducible factor-1α overexpression indicates poor clinical outcomes in tongue squamous cell carcinoma.

The aim of the present study was to investigate the expression of hypoxia-inducible factor-1α (HIF-1α) in tongue squamous cell carcinoma (TSCC) and to assess its possible impact on prognosis. A total of 49 tumor samples and 15 adjacent non-tumor samples from 49 patients treated between January 2000 and December 2005 at the Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Tongji University (Shanghai, China) were obtained for investigation with immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR). The expression of HIF-1α was detected in 87.

Effects of trichostatin A on HIF-1α and VEGF expression in human tongue squamous cell carcinoma cells in vitro.

Hypoxia is an essential feature of the microenvironment of solid tumors, which regulates a variety of transcription factors including hypoxia-inducible factor-1α (HIF-1α). HIF-1α overexpression enhances tumor angiogenesis via upregulation of vascular endothelial growth factor (VEGF) and some other hypoxia-inducible angiogenic factors, which lead to a more aggressive tumor phenotype, tumor metastasis and resistance to radiation and chemotherapy. In this study, we found that a histone deacetylase (HDAC) inhibitor, trichostatin A (TSA), inhibited cell proliferation and invasion, blocked the cell cycle, and induced cell apoptosis in a dose- and time-dependent manner in the human tongue squamous cell carcinoma (TSCC) SCC-6 cell line in vitro.

[Biological characteristics of CD133+ subpopulation in tongue squamous cell carcinoma Tca8113 cell line].

Objective: To detect the expression of CD133 in human tongue squamous cell carcinoma Tea8113 cell line and observe proliferation ability of CD133 groups in vitro.

Methods: Limiting dilution assay was employed to observe the proliferating character of Tca8113 single cell in vitro. The ability of growing as cancer spheroids was observed with ultra-low attachment plates.

[Proteomics study of the serum biomarkers in tongue cancer].

Objective: To search for specific serum biomarkers associated with tongue cancer by means of the serum proteomics technology.

Methods: The tongue cancer cells of human tongue cancer cell line Tca8113 were subcutaneously inoculated into nude mice, while control nude mice were injected with phosphate-buffered saline. Serums from these two group of mice were collected for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS).

[Experimental studies on ectopic osteogenesis by seeding human mesenchymal stem cell on human natural bone derived scaffold materials].

Objective: To investigate the ectopic osteogenesis potential of human natural bone derived material combined with human bone marrow mesenchymal stem cells (MSCs).

Methods: Cell-scaffold complexes were implanted subcutaneously into the left back of the nude mice, and human natural bone derived material were implanted into the right back as control group. The mice were killed respectively on the postoperative 2 weeks, 4 weeks and 8 weeks.