PI(4,5)P2 alleviates colitis by inhibiting intestinal epithelial cell pyroptosis through NNMT-mediated RBP4 m6A modification.

Lipid metabolism disorder is a critical feature of Crohn's disease (CD). Phosphatidylinositol (PI) and its derivative, phosphatidylinositol bisphosphate (PIP2), are associated with CD. The mechanisms underlying such association remain unknown.

Insights into the divergence of the photosynthetic LH1 complex obtained from structural analysis of the unusual photocomplexes of Roseospirillum parvum.

Purple phototrophic bacteria produce two kinds of light-harvesting complexes that function to capture and transmit solar energy: the core antenna (LH1) and the peripheral antenna (LH2). The apoproteins of these antennas, encoded respectively by the genes pufBA and pucBA within and outside the photosynthetic gene cluster, respectively, exhibit conserved amino acid sequences and structural topologies suggesting they were derived from a shared ancestor. Here we present the structures of two photosynthetic complexes from Roseospirillum (Rss.

Cardiovascular Events Associated with CDK4/6 Inhibitors: A Safety Meta-Analysis of Randomized Controlled Trials and a Pharmacovigilance Study of the FAERS Database.

Background: CDK4/6 inhibitors are highly valued, but the incidence of cardiovascular adverse events (CVAEs) associated with CDK4/6 inhibitors is not clear.

Objective: Our aim was therefore to assess the risk of developing CVAEs associated with CDK4/6 inhibitors, by conducting a systematic review and meta-analysis of randomized controlled trials (RCTs), along with a pharmacovigilance study of the FDA Adverse Event Reporting System (FAERS) database.

Methods: Eligible CVAEs were extracted from the ClinicalTrials.

Effective xylan integration for remodeling biochar uniformity and porosity to enhance chemical elimination and CO adsorption.

Although plant evolution has offered diverse biomass resources, the production of high-quality biochar from desirable lignocelluloses remains unexplored. In this study, distinct lignocellulose substrates derived from eight representative plant species were employed to prepare biochar samples under three different temperature treatments. Correlation analysis showed that only hemicellulose was a consistently positive factor of lignocellulose substrates to account for the dye-adsorption capacities of diverse biochar samples.

NF-κB/Relish readjusts miR-100 expression and recovers immune homeostasis in Drosophila melanogaster.

The regulation and maintenance of immune homeostasis are essential for animal survival, but the molecular mechanisms are not fully understood. Here, we used the model organism Drosophila melanogaster to uncover a potential mechanism by which the nuclear factor-κB transcription factor Relish and miR-100 cooperatively regulate innate immune homeostasis. We first demonstrated in vitro and in vivo that miR-100 can negatively regulate the immune responses of the Imd pathway by inhibiting the expression of TAK1-associated binding protein 2 (Tab2) gene.

Attenuated Total Reflectance Crystal of Silicon for Rapid Nitrate Sensing Combining Mid-Infrared Spectroscopy.

Rapid detection of NO -N is critical to address the challenges of food security, environmental degradation, and climate change. Conventional methods for sensing NO -N in water demand pretreatments and chemical reagents, which are time- and cost-consuming. Consequently, Fourier transform infrared attenuated total reflectance (FTIR-ATR) spectroscopy has been well applied for the determination of NO -N.

HBSP: a hybrid bilinear and semidefinite programming approach for aligning partially overlapping point clouds.

In many applications, there is a need for algorithms that can align partially overlapping point clouds while remaining invariant to corresponding transformations. This research presents a method that achieves these goals by minimizing a binary linear assignment-least squares (BLALS) energy function. First, we reformulate the BLALS problem as the minimization of a quadratic function with quadratic and linear constraints through variable substitution.

A Cross-Sectional Study on the Association between Physical Activity and Cognitive Function Among Community-Dwelling Older Adults in Tianjin.

Background: The association between physical activity (PA) and cognitive function remains controversial, and the impact of gender on this association remains underexplored. Therefore, this study aimed to investigate the association between PA and cognitive function and to explore whether this association was modified by gender among older adults.

Methods: In 2016, a cluster sampling method was used to select community-dwelling older adults aged 65 and above.

Itaconic acid ameliorates necrotizing enterocolitis through the TFEB-mediated autophagy-lysosomal pathway.

Excessive autophagy has been implicated in the pathogenesis of necrotizing enterocolitis (NEC), yet the molecular underpinnings of the autophagy-lysosomal pathway (ALP) in NEC are not well characterized. This study aimed to elucidate alterations within the ALP in NEC by employing RNA sequencing on intestinal tissues obtained from affected infants. Concurrently, we established animal and cellular models of NEC to assess the therapeutic efficacy of itaconic acid (ITA).

Endogenous Telomerase-Activated Fluorescent Probes for Specific Detection and Imaging of Flap Endonuclease 1 in Cancer Cells and Tissues.

Flap endonuclease 1 (FEN1) is a structure-specific DNA repair enzyme that has emerged as a potential target for cancer diagnosis and treatment. However, existing FEN1 assays often suffer from complicated reaction schemes and laborious procedures, and only a few methods are available for the detection and imaging of FEN1 in living cells. Especially, FEN1 is not exclusive to cancer cells, but it is also shared by normal cells.

Everolimus treatment in patients with hormone receptor-positive and human epidermal growth factor receptor 2-negative advanced breast cancer and a predictive model for its efficacy: a multicenter real-world study.

Background: Everolimus is beneficial for patients with hormone receptor-positive and human epidermal growth factor receptor 2-negative (HR+/HER2-) advanced breast cancer (ABC). However, some patients developed drug resistance and the well-established predictor for everolimus efficacy was limited.

Objectives: The study was designed to evaluate the efficacy of everolimus in different treatment lines and identify several clinicopathological markers to estimate everolimus efficacy in patients with HR+/HER2- ABC.

Modulation of persistent bladder pain in mice: The role of macrophage migration inhibitory factor, high mobility group box-1, and downstream signaling pathways.

Background: Repeated intravesical activation of protease-activated receptor-4 (PAR4) serves as a model of persistent bladder hyperalgesia (BHA) in mice, which lasts several days after the final stimulus. Spinal macrophage migration inhibitory factor (MIF) and high mobility group box 1 (HMGB1) are critical mediators in the persistence of BHA.

Objective: We aimed to identify effective systemic treatments for persistent BHA using antagonists or transgenic deletions.

Static-spun mesoporous silica-coated CsPbBr blue fibres: synthesis and fluorescence properties.

Due to their excellent properties, blue CsPbBr quantum dots show great promise for full-colour display and lighting applications. This study used acetonitrile, a polar solvent, to post-treat CsPbBr quantum dots, resulting in a blue shift to 453 nm. To enhance stability, these quantum dots were encapsulated within the pore structure of mesoporous silica.

SFNet: Spatial and Frequency Domain Networks for Wide-Field OCT Angiography Retinal Vessel Segmentation.

Automatic segmentation of blood vessels in fundus images is important to assist ophthalmologists in diagnosis. However, automatic segmentation for Optical Coherence Tomography Angiography (OCTA) blood vessels has not been fully investigated due to various difficulties, such as vessel complexity. In addition, there are only a few publicly available OCTA image data sets for training and validating segmentation algorithms.

Impaired inducibility of immune regulatory capacity of peripheral B cells of patients with recurrent pregnancy loss.

The pathogenesis of recurrent pregnancy loss (RPL) is unclear. RPL may have an association with disruption of immune tolerance. The aim of this study is to characterize the inducibility of immune regulatory ability in peripheral naïve B cells of patients with RPL.

Comparative efficacy of anthracycline-free and anthracycline-containing neoadjuvant chemoimmunotherapy regimens for triple-negative breast cancer.

Background: The selection of appropriate chemotherapy backbone agents in combination with neoadjuvant immunotherapy for triple-negative breast cancer (TNBC) remains unclear. Herein, we aimed to evaluate the efficacy and safety of anthracycline-free and anthracycline-containing regimens coupled with neoadjuvant immunotherapy.

Method: This retrospective study included 87 patients with TBNC who received neoadjuvant immunotherapy combined with various chemotherapy regimens at three research centers from November 2020 to November 2023.

MIKC*-type MADS transcription factors control JINGUBANG expression and the degree of pollen dormancy in Arabidopsis.

While pollen dormancy has been proposed to play a necessary role in sexual reproduction, it remains poorly understood. Here, we used traditional pollen germination assays to characterize dormancy. Our results underscore variation in the degree of dormancy between individual pollen grains.

Ultrafast Room-Temperature Synthesis of Phosphate-Intercalated NiFe Layered Double Hydroxides for High-Performance Alkaline Seawater Oxidation.

Quick and easy synthetic methods and highly efficient catalytic performance are equally important to anodic oxygen evolution reaction (OER) electrocatalysts for alkaline seawater electrolysis. Herein, we report a facile one-step route to in situ growing PO intercalated NiFe layered double hydroxides (NiFe-LDH) on Ni foam (denoted as NiFe-P/NF) by a room-temperature immersion for several minutes. This ultrafast approach transforms the NF surface into a rough PO intercalated NiFe-LDH overlayer, which demonstrates outstanding OER performance in both alkaline simulated and natural seawaters owing to good hydrophilic interface and the electrostatic repulsion of PO against Cl anions.