Soybean aphid (Hemiptera: Aphididae) development on soybean with Rag1 alone, Rag2 alone, and both genes combined.

Aphis glycines Matsumura (Hemiptera: Aphididae) can reduce the yield of aphid-susceptible soybean (Glycine max (L.) Merrill) cultivars. The Rag1 and Rag2 genes conferresistance to some biotypes of A.

Effect of seed development stage on sphingolipid and phospholipid contents in soybean seeds.

Glucosylceramide (GlcCer) and ceramide (Cer) are the predominant sphingolipids (SL) in soybeans. They have been recognized as functional components in plants and may have health benefits for humans. The objective of this study was to evaluate the changes in SL and phospholipid (PL) contents that occurred during seed development.

HPLC quantification of sphingolipids in soybeans with modified palmitate content.

Efficient separation and accurate quantification of sphingolipids (SL) are important for studying SL concentrations and biological functions. The objectives of this study were to develop effective methods for the separation and quantification of SL and to determine the relationship between palmitate and SL contents of mature soybean seeds. Methods using column chromatography and high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) were developed to separate and quantify glucosylceramide (GlcCer) and ceramide (Cer) in 15 soybeans lines in which palmitate content ranged from 3.

Total and water-soluble phosphorus excretion from swine fed low-phytate soybeans.

A study was conducted to evaluate the impact of feeding soybean meal (SBM) from low-phytate (LP) or traditional phytate (TP) soybeans on performance and excretions from growing swine. Ninety-six crossbred barrows (initial BW = 18 +/- 0.3 kg) were allocated by BW to 24 pens and fed 1 of 4 treatment diets: TP SBM without supplemental phytase; TP SBM plus 500 phytase units of phytase/kg, as-fed basis [Ronozyme P (CT) 2500; DSM Nutritional Products, Basel, Switzerland]; LP SBM (USDA-ARS breeding line CX1834-1) without supplemental phytase, and LP SBM plus phytase.

Functional profiling of mercuric reductase (mer A) genes in biofilm communities of a technical scale biocatalyzer.

Background: Bacterial mercury resistance is based on enzymatic reduction of ionic mercury to elemental mercury and has recently been demonstrated to be applicable for industrial wastewater clean-up. The long-term monitoring of such biocatalyser systems requires a cultivation independent functional community profiling method targeting the key enzyme of the process, the merA gene coding for the mercuric reductase. We report on the development of a profiling method for merA and its application to monitor changes in the functional diversity of the biofilm community of a technical scale biocatalyzer over 8 months of on-site operation.

The microbial diversity in picoplankton enrichment cultures: a molecular screening of marine isolates.

Picoplankton bacteria from a North Sea water sample were cultured under a variety of different conditions (nutrients, temperature, light, agitation, adhesion). Fluorescent in situ hybridization (FISH) analysis of the enrichments showed complex communities which were dominated by gamma-Proteobacteria or beta-Proteobacteria, followed by alpha-Proteobacteria and bacteria from the Cytophaga/Flavobacterium/Bacteroides (CFB) cluster. Among 410 isolates, a high degree of diversity was found, both with respect to colony color and morphology and with respect to genetic diversity.

Biofilm community structure in polluted rivers: abundance of dominant phylogenetic groups over a complete annual cycle.

The seasonal dynamics of river biofilm communities in two German rivers, the Elbe and one of its tributaries, the Spittelwasser, were investigated for the first time by using fluorescence in situ hybridization and a standardized biofilm sampling procedure. We show the importance of members of the beta subclass of the class Proteobacteria, which formed the largest single group in the massively polluted Spittelwasser at all times. Clear seasonal peaks of abundance were observed for the planctomycetes and the Cytophaga-Flavobacterium cluster.

Genetic variation for quantitative traits in soybean lines derived from tissue culture.

Tissue culture may generate useful genetic variation for quantitative traits. The objective of this study was to analyze genetic variation for ten quantitative traits of soybean [Glycine max (L.) Merr.

RFLP analysis of soybean seed protein and oil content.

The objectives of this study were to present an expanded soybean RFLP map and to identify quantitative trait loci (QTL) in soybean [Glycine max (L.) Merr.] for seed protein and oil content.