The 2025 Motile Active Matter Roadmap.

Activity and autonomous motion are fundamental aspects of many living and engineering systems. Here, the scale of biological agents covers a wide range, from nanomotors, cytoskeleton, and cells, to insects, fish, birds, and people. Inspired by biological active systems, various types of autonomous synthetic nano- and micromachines have been designed, which provide the basis for multifunctional, highly responsive, intelligent active materials.

Low efficiency of Janus microswimmers as hydrodynamic mixers.

The generation of fluid flows by autophoretic microswimmers has been proposed as a mechanism to enhance mass transport and mixing at the micro- and nanoscale. Here, we experimentally investigate the ability of model 2D active baths of photocatalytic silica-titania Janus microspheres to enhance the diffusivity of tracer particles at different microswimmer densities below the onset of collective behavior. Inspired by the similarities between our experimental findings and previous results for biological microorganisms, we then model our Janus microswimmers using a general squirmer framework, specifically treating them as neutral squirmers.

Chloroplast Genome Variation and Phylogenetic Relationships of Autochthonous Varieties of from the Don Valley.

The autochthonous grape varieties of the Don Valley, situated in southern Russia, constitute a distinctive element of regional cultural heritage. These varieties have been adapted over centuries to the region's specific local climatic and soil conditions. For the most part, these varieties are not imported from other countries.

Collective behavior of squirmers in thin films.

Bacteria in biofilms form complex structures and can collectively migrate within mobile aggregates, which is referred to as swarming. This behavior is influenced by a combination of various factors, including morphological characteristics and propulsive forces of swimmers, their volume fraction within a confined environment, and hydrodynamic and steric interactions between them. In our study, we employ the squirmer model for microswimmers and the dissipative particle dynamics method for fluid modeling to investigate the collective motion of swimmers in thin films.

Motion of microswimmers in cylindrical microchannels.

Biological and artificial microswimmers often have to propel through a variety of environments, ranging from heterogeneous suspending media to strong geometrical confinement. Under confinement, local flow fields generated by microswimmers, and steric and hydrodynamic interactions with their environment determine the locomotion. We propose a squirmer-like model to describe the motion of microswimmers in cylindrical microchannels, where propulsion is generated by a fixed surface slip velocity.

Assessing Differential Particle Deformability under Microfluidic Flow Conditions.

Assessing the mechanical behavior of nano- and micron-scale particles with complex shapes is fundamental in drug delivery. Although different techniques are available to quantify the bulk stiffness in static conditions, there is still uncertainty in assessing particle deformability in dynamic conditions. Here, a microfluidic chip is designed, engineered, and validated as a platform to assess the mechanical behavior of fluid-borne particles.

Dynamic shapes of floppy vesicles enclosing active Brownian particles with membrane adhesion.

Recent advances in micro- and nano-technologies allow the construction of complex active systems from biological and synthetic materials. An interesting example is active vesicles, which consist of a membrane enclosing self-propelled particles, and exhibit several features resembling biological cells. We investigate numerically the behavior of active vesicles, where the enclosed self-propelled particles can adhere to the membrane.

Competition between deformation and free volume quantified by 3D image analysis of red blood cell.

Cells in living organisms are subjected to mechanical strains caused by external forces like overcrowding, resulting in strong deformations that affect cell function. We study the interplay between deformation and crowding of red blood cells (RBCs) in dispersions of nonabsorbing rod-like viruses. We identify a sequence of configurational transitions of RBC doublets, including configurations that can only be induced by long-ranged attraction: highly fluctuating T-shaped and face-to-face configurations at low, and doublets approaching a complete spherical configuration at high, rod concentrations.

Hydrodynamic clustering of two finite-length flagellated swimmers in viscoelastic fluids.

Clustering of flagellated microswimmers such as sperm is often mediated by hydrodynamic interactions between them. To better understand the interaction of microswimmers in viscoelastic fluids, we perform two-dimensional simulations of two swimming sheets, using a viscoelastic version of the smoothed dissipative particle dynamics method that implements the Oldroyd-B fluid model. Elasticity of sheets (stiff versus soft) defines two qualitatively different regimes of clustering, where stiff sheets exhibit a much more robust clustering than soft sheets.

Benchmarking the quadrupolar coupling tensor for chlorine to probe weak-bonding interactions.

Rotational spectroscopy relies on quantum chemical calculations to interpret observed spectra. Among the most challenging molecules to assign are those with additional angular momenta coupling to the rotation, contributing to the complexity of the spectrum. This benchmark study of computational methods commonly used by rotational spectroscopists targets the nuclear quadrupole coupling constants of chlorine containing molecules and the geometry of its complexes and clusters.

Correction: Non-equilibrium shapes and dynamics of active vesicles.

Correction for 'Non-equilibrium shapes and dynamics of active vesicles' by Priyanka Iyer , , 2022, , 6868-6881, https://doi.org/10.1039/D2SM00622G.

Non-equilibrium shapes and dynamics of active vesicles.

Active vesicles, constructed through the confinement of self-propelled particles (SPPs) inside a lipid membrane shell, exhibit a large variety of non-equilibrium shapes, ranging from the formation of local tethers and dendritic conformations, to prolate and bola-like structures. To better understand the behavior of active vesicles, we perform simulations of membranes modelled as dynamically triangulated surfaces enclosing active Brownian particles. A systematic analysis of membrane deformations and SPP clustering, as a function of SPP activity and volume fraction inside the vesicle is carried out.

Erythrocyte sedimentation: Effect of aggregation energy on gel structure during collapse.

The erythrocyte (or red blood cell) sedimentation rate (ESR) is commonly interpreted as a measure of cell aggregation and as a biomarker of inflammation. It is well known that an increase of fibrinogen concentration, an aggregation-inducing protein for erythrocytes, leads to an increase of the sedimentation rate of erythrocytes, which is generally explained through the formation and faster settling of large disjoint aggregates. However, many aspects of erythrocyte sedimentation conform well with the collapse of a particle gel rather than with the sedimentation of disjoint aggregates.

Erythrocyte Sedimentation: Collapse of a High-Volume-Fraction Soft-Particle Gel.

The erythrocyte sedimentation rate is one of the oldest medical diagnostic methods whose physical mechanisms remain debatable today. Using both light microscopy and mesoscale cell-level simulations, we show that erythrocytes form a soft-particle gel. Furthermore, the high volume fraction of erythrocytes, their deformability, and weak attraction lead to unusual properties of this gel.

SNP-Based Analysis Reveals Authenticity and Genetic Similarity of Russian Indigenous Grape Cultivars.

9 Russian grape varieties and the European variety Muscat Hamburg were sequenced and genotyped using 527 SNPs (single nucleotide polymorphisms) with high minor allele frequency for the first time. The data were coupled with previously identified genotypes of 783 varieties and subjected to parentage and population analysis. As a result, contrary to the historical and ampelographic data published in many sources from 1800 to 2012, only two of the nine Russian varieties (Pukhlyakovskiy Belyi and Sibirkovyi) were related to foreign ones and were obviously imported from Europe to the Russian Empire.

Effect of malaria parasite shape on its alignment at erythrocyte membrane.

During the blood stage of malaria pathogenesis, parasites invade healthy red blood cells (RBC) to multiply inside the host and evade the immune response. When attached to RBC, the parasite first has to align its apex with the membrane for a successful invasion. Since the parasite's apex sits at the pointed end of an oval (egg-like) shape with a large local curvature, apical alignment is in general an energetically unfavorable process.

The Erythrocyte Sedimentation Rate and Its Relation to Cell Shape and Rigidity of Red Blood Cells from Chorea-Acanthocytosis Patients in an Off-Label Treatment with Dasatinib.

Background: Chorea-acanthocytosis (ChAc) is a rare hereditary neurodegenerative disease with deformed red blood cells (RBCs), so-called acanthocytes, as a typical marker of the disease. Erythrocyte sedimentation rate (ESR) was recently proposed as a diagnostic biomarker. To date, there is no treatment option for affected patients, but promising therapy candidates, such as dasatinib, a Lyn-kinase inhibitor, have been identified.

Acanthocyte Sedimentation Rate as a Diagnostic Biomarker for Neuroacanthocytosis Syndromes: Experimental Evidence and Physical Justification.

(1) Background: Chorea-acanthocytosis and McLeod syndrome are the core diseases among the group of rare neurodegenerative disorders called neuroacanthocytosis syndromes (NASs). NAS patients have a variable number of irregularly spiky erythrocytes, so-called acanthocytes. Their detection is a crucial but error-prone parameter in the diagnosis of NASs, often leading to misdiagnoses.

Effect of cytosol viscosity on the flow behavior of red blood cell suspensions in microvessels.

Objective: The flow behavior of blood is strongly affected by red blood cell (RBC) properties, such as the viscosity ratio C between cytosol and suspending medium, which can significantly be altered in several pathologies (e.g. sickle-cell disease, malaria).

Active particles induce large shape deformations in giant lipid vesicles.

Biological cells generate intricate structures by sculpting their membrane from within to actively sense and respond to external stimuli or to explore their environment. Several pathogenic bacteria also provide examples of how localized forces strongly deform cell membranes from inside, leading to the invasion of neighbouring healthy mammalian cells. Giant unilamellar vesicles have been successfully used as a minimal model system with which to mimic biological cells, but the realization of a minimal system with localized active internal forces that can strongly deform lipid membranes from within and lead to dramatic shape changes remains challenging.

Deterministic Lateral Displacement: Challenges and Perspectives.

The advent of microfluidics in the 1990s promised a revolution in multiple industries from healthcare to chemical processing. Deterministic lateral displacement (DLD) is a continuous-flow microfluidic particle separation method discovered in 2004 that has been applied successfully and widely to the separation of blood cells, yeast, spores, bacteria, viruses, DNA, droplets, and more. Deterministic lateral displacement is conceptually simple and can deliver consistent performance over a wide range of flow rates and particle concentrations.

Stochastic bond dynamics facilitates alignment of malaria parasite at erythrocyte membrane upon invasion.

Malaria parasites invade healthy red blood cells (RBCs) during the blood stage of the disease. Even though parasites initially adhere to RBCs with a random orientation, they need to align their apex toward the membrane in order to start the invasion process. Using hydrodynamic simulations of a RBC and parasite, where both interact through discrete stochastic bonds, we show that parasite alignment is governed by the combination of RBC membrane deformability and dynamics of adhesion bonds.

Dissipative particle dynamics with energy conservation: Isoenergetic integration and transport properties.

Simulations of nano- to micro-meter scale fluidic systems under thermal gradients require consistent mesoscopic methods accounting for both hydrodynamic interactions and proper transport of energy. One such method is dissipative particle dynamics with energy conservation (DPDE), which has been used for various fluid systems with non-uniform temperature distributions. We propose an easily parallelizable modification of the velocity-Verlet algorithm based on local energy redistribution for each DPDE particle such that the total energy in a simulated system is conserved up to machine precision.