[Results of studies of human dirofilariasis in Russia].

Human dirofilariasis is a pressing health problem in Russia. By 2014, there have been as many as 850 Dirofilaria repens-infested people living in 42 subjects of the Russian Federation. One of the favorable factors for circulation and spread of invasion is a temperature of above +20-24 degrees C; when the latter is maintained during at least 20 days there may be 1.

[Pseudoinvasion and transient carriage of the larvae of arthropods and soil nematodes in humans: problems and consequences. The authors' observations and a literature review].

The paper describes cases of pseudoinvasion and transient carriageof the larvae of arthropods and geohelminths. It highlights the objective and subjective factors in the diagnosis of arthropod-borne diseases and geohelminthiasis, which give rise to misdiagnosis. Distinctions between real invasion, transient invasion, and pseudoinvasion are pointed out.

[Morphological identification of blastocystis].

The authors have attempted to systematize the currently known specific morphological features of the composition of Blastocystis spp. existing in different forms and to present this material as a reference table, by understanding the need for further data clarification. In addition, the paper describes observations of variations in the forms of human blastocysts.

[Blastocystis spp. culturing as a method for diagnosing the parasite].

The technique for isolating blastocysts in the culture was assessed, by examining the fresh specimens from 196 patients with gastrointestinal tract (GIT) disorders and from 56 persons with an uncertain protozoological diagnosis. A set of techniques, such as triple examination of native smears, parallel inoculation of the Pavlova medium, and microscopy of obtained permanent specimens as may be required, contributes to the timely identification of protozoa, including blastocysts, in persons with GIT disorder. The efficiency of the technique for isolating blastocysts on Pavlova's medium and the native smear test averaged 34.

[Experimental study on impact of salmon milt DNA on T-cellular immunity].

The impact of a biologically active food supplement containing salmon milt DNA on cellular immunity was experimentally studied. It was shown that the DNA had a dose-dependent stimulating effect on lymphocyte transformation, stimulated the DTH response and protected from experimental listeriosis infection.

[Experimental study on the effect of low molecular DNA from salmon milt on hemopoiesis].

Biological activity of low molecular DNA isolated from salmon milt was studied. When administered subcutaneously to mice with with acute experimental radiation disease in a course dose of 10 mg/kg, it showed a therapeutic effect, stimulated hemopoiesis, increased the survival rate and the average life span of the animals. Moreover, its marked effect on the humoral immunity was observed.

[Efficacy of the Russian anthelmintic agent trichlorophen].

Experiments have established the high efficacy of combinations of the micronized dosage form of trichlorophen, with albendazole or medamine in treating trichocephaliasis (its causative agent being Trichocephalus muris) in DBA/2st mice and that of trichlorophen in combination with azinox or fenasal in outbred albino mice with hymenolepiasis (its causative agent being Hymenolepis nana). These combinations are promising in treating patients with cestodosis and nemadosis, respectively.

[Activity of the anthelmintic agent trichlorophen on the models of human helminthiasis].

Trials of trichlorophen have shown its high efficacy on models of cestode infections: hymenolepiasis (at the adult and cysticercoid stages of development on three types of animals: outbred albino mice, albino rats and golden hamsters), preimaginal echinococciasis alveolaris, larval alveolar echinococciasis (at the early stage of development of the parasite in experiments on cotton rats). The high nematodical activity of trichlorophen was first found on models of trichocephaliasis in DBA/2y mice, nippostrongyloidiasis (in in vitro experiments), and aspiculuriasis in outbred mice. The agent proved to be ineffective at the tissue developmental stage of Hymenolepsis nana (H.

[The new agent G-1724: synthesis and test on murine models of trichuriasis and trichinosis].

To search for new antihelminthics, the new compound N-[3-chloro-4-[(1-ethyl-2-benzimidazolyl) thio]phenyl]-2-hydroxy-3,5-dibromobenzamide (the agent G-1724) was synthesized and tested by using the models of trichocephaliasis (Trichocephalus muris) and trichinosis (Trichinella spiralis). The tests demonstrated that this agent had an nematocidal effect (71-78%).

[Tanfelam: synthesis, study of ovicidal activity, and acute toxicity].

The papers describes the synthesis of N-(2-piperidinoethyl)-N-tosyl-n-anisidine (Tanfelam) which showed a 100% ovicidal activity when tested by the Harada and Mori methods (in vitro inhibited N.brasiliensis hatching and development test). Tanfelam has been transferred for in-depth tests.