Publications by authors named "Federica Daniele"
Article Synopsis
- Mutations in the LRRK2 gene are linked to familial Parkinson's disease, with the protein having important interaction domains at both its ends.
- This study used microscopy and assays to find that the N- and C-terminal domains of LRRK2 affect synaptic vesicle movement in opposing ways and bind to different proteins.
- The G2385R variant in the C-terminal domain increases Parkinson's risk by altering protein interactions and synaptic vesicle fusion, potentially leading to impaired vesicular trafficking and disease progression.
Synaptic vesicles release neurotransmitters at chemical synapses through a dynamic cycle of fusion and retrieval. Monitoring synaptic activity in real time and dissecting the different steps of exo-endocytosis at the single-vesicle level are crucial for understanding synaptic functions in health and disease. Genetically-encoded pH-sensitive probes directly targeted to synaptic vesicles and Total Internal Reflection Fluorescence Microscopy (TIRFM) provide the spatio-temporal resolution necessary to follow vesicle dynamics.
View Article and Find Full Text PDFXenopus laevis oocytes are an interesting model for the study of many developmental mechanisms because of their dimensions and the ease with which they can be manipulated. In addition, they are widely employed systems for the expression and functional study of heterologous proteins, which can be expressed with high efficiency on their plasma membrane. Here we applied atomic force microscopy (AFM) to the study of the plasma membrane of X.
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