[Determination of the rate of utilization of glucose in a suspension of erythrocytes].

The authors suggest a new method for measuring the rate of glucose utilization in a cell (red cell) suspension, making use of interferometry. This method allows an easy examination of glucose utilization kinetics in time with but 1000 cells. Thermostatic control of the sample in the cuvette of the device permits detecting the temperature effects on glucose metabolism kinetics and thus define the temperature optimal for the process.

[Solid-phase immunoenzyme analysis of apoprotein A-1].

The authors have optimized indirect solid-phase enzyme immunoassay for measuring human blood serum apoA-1. Immunochemical identification of the obtained apoA-1 and specific antibodies has been carried out, working regimens for the components and conjugates employed have been selected. Relationship between the method of preliminary treatment of the serum and the completeness of detected apoA-1 antigenic determinants is demonstrated.

[Rheological properties of erythrocyte suspensions in acute myocardial infarct in the physiological temperature range].

A study was made over time of the relationships between the viscosity of red cell suspensions and temperature [eta (T)] in normal subjects and patients with acute myocardial infarction. In normal subjects, the curves eta (T) have a bend within the temperature range 37-40 degrees C, which apparently reflects the phasic transition. In patients with myocardial infarction, the bend on the curve eta (T) either disappears or is undemonstrable during the acute period within the temperature range indicated.

[Mechanisms of the structural changes in erythrocyte membranes under the action of a shift in pH].

The effect of pH shifts (5--10) on erythrocyte "ghosts" has been studied using the IR spectroscopy. The process of regulation has been shown to take place within pH 7.15--8 range involving both protein (transition testified by absorption band changes 1520, 1540, 1635, 1645, 1585, 1696, 3305 CM-1) and lipid membrane components testified by absorption band splits 1745, 1470, 1380, 720 AND 1242 cm-1.

[Microthermal method of separating red cells into groups according to age].

The microthermal method of red blood cells' division according to their age is based on the formation of a temperature gradient with the aid of microthermoresistor. The red blood cells of different age fractions are consecutively localized and selected in the zone of microthermoresistor. They differ from one another according to their biochemical indices.