Mutational analysis of cytoplasmic domain of integrin subunit alpha-1 and its association with periapical wound healing after surgical endodontic treatment.

Background: Numerous studies reported that the healing after surgical endodontic retreatment is influenced by multiple factors which include the genetic profile of the patient, epigenetics, and immune responses. The genes which are primarily responsible for the healing potential in different individuals are those which are involved in the regulation of the cytoskeleton and cellular adhesion which subsequently affects bone deposition and healing. Integrins are cell-surface molecules, possess a key role in the cytoskeleton and cellular adhesion.

Investigating In Situ Expression of Neurotrophic Factors and Partner Proteins in Irreversible Pulpitis.

Introduction: In situ assessments of neurotrophic factors and their associated molecular partners have not been explored to date, particularly in humans. The present investigation aimed to explore the expressional dysregulation of neurotrophic factors (nerve growth factor [NGF], brain derived neurotrophic factor [BDNF], and NT4/5), their receptors (TrkA and TrkB), and their modulators (USP36 and Nedd4-2) directly in irreversibly inflamed human pulp tissues.

Methods: Forty samples each of healthy and irreversibly inflamed pulp were extirpated for the study.

Comparative evaluation of Sapindus Mukorossi extract with 17% EDTA on smear layer removal in various parts of the tooth root.

Objectives: This study aimed to compare the effectiveness of an experimental root canal irrigant and 17% Ethylene-di-amine tetra acetic acid for removal of the smear layer in the coronal, middle and apical portions of the root canal.

Materials And Methods: Ninety human single rooted maxillary and mandibular teeth were selected for this study. The teeth were randomly divided into two experimental groups and one control group as follows: Group A (Ethanolic extract of Sapindus Mukorossi), Group B (17% EDTA), and Group C (Distilled water).

Effect of Erosive Agents on Surface Characteristics of Nano-Fluorapatite Ceramic: An In-Vitro Study.

Erosive beverages cause dissolution of natural teeth and intra-oral restorations, resulting in surface characteristic changes, particularly roughness and degradation. The purpose of this study was to evaluate the surface roughness and topography of a dental ceramic following immersion in locally available erosive solutions. A total of 160 disc specimens of a nano-fluorapatite type ceramic (12 mm diameter and 2 mm thickness) were fabricated and equally distributed into two groups ( = 80) and then evenly distributed among the following five testing groups ( = 16): lemon juice, citrate buffer solution, 4% acetic acid, soft cola drink, and distilled water which served as a control.

An In-Vitro Evaluation of Microleakage in Resin-Based Restorative Materials at Different Time Intervals.

A vital feature of conservative dentistry is the adhesion of the restorative material to the tooth structure for restoration of the tooth substance lost due to dental decay, trauma, or dental imperfections. In a perfect world, a restorative material should generate a lasting adhesion by bonding the restoration with tooth tissues. The ingress of micro-organisms, oral fluids, molecules, and ions through microscopic spaces due to faulty adhesion between restoration and tooth structure is known as microleakage.

Comparison of gingival biotype in smokeless tobacco users (Gutka and Paan) and non-tobacco users.

Objective: To assess the gingival biotypes in smokeless tobacco (Gutka and Paan) users and compare it with non-tobacco users in Karachi sub-population using trans-gingival probing method (TRAN).

Methods: This in-vivo, cross sectional study was conducted in the department of Operative Dentistry from 20th February 2019 to 25th June 2019 Dow University of Health Sciences, Karachi after obtaining ethical approval from the Institutional review board of DUHS (Ref: IRB-1207/DUHS/Approval/2019/21). A total of 70 participants, 35 subjects currently using smokeless tobacco (Gutka and Paan) and 35 non-tobacco users from both genders were included in the study after taking informed consent.

Effect of pre-cooling agent on intensity of pricking pain at intraoral injection site in adults: An experimental study.

Objective: To determine the effect of pre-cooling agent on the intensity of pricking pain at the intraoral injection site in adult patients.

Methods: The in-vivo interventional study was conducted at the Department of Operative Dentistry, Dr. Ishrat-ul-Ebad Khan Institute of Oral Health Sciences, Dow University of Health Sciences, Karachi, from September 2018 to August 2019, and comprised adult patients of either gender.

Erosive Potential of 1% Phytic Acid on Radicular Dentine at Different Time Intervals.

Objective: The objective of this in-vitro study was to compare the erosive potential and smear layer removal ability of 1% Phytic acid (IP6) and 17% Ethylenediaminetetaacetic acid (EDTA).

Methods: Canal preparation of 225 single rooted extracted human teeth was performed with Protaper NiTi rotary instruments. Teeth were divided into three groups according to the final irrigation protocol.

Audiovisual Distraction: A Pricking Pain Reduction Modality among Ladies Receiving Intraoral Injections.

Objective: To determine the effect of audiovisual distraction (AVD) using virtual reality device (bobo VRZ4-VRBOX) on intensity of pricking pain at the intraoral injection site among different age groups in ladies.

Study Design: Experimental study.

Place And Duration Of Study: Department of Operative Dentistry, Dr.

An In-VitroEvaluation of Microleakage at the Cervical Margin Between two Different Class II Restorative Techniques Using Dye Penetration Method.

Objective: To evaluate the coronal microleakage of packable composite using conventional incremental and posterior bulk fill flowable composite using Smart Dentine Replacement Single Step technique in the cervical margins of class II cavities in dentine using the dye penetration method.

Study Design: In-vitroInterventional study.

Place And Duration Of Study: Department of Operative Dentistry, Dr.

Micromechanical Intervention in Sandwich Restoration.

Objective: To evaluate the sealing ability of two different types of Glass Ionomer Cements (GICs) used for sandwich restorations and assess the effect of acid etching of GIC on microleakage at GIC-resin composite interface.

Study Design: Experimental study.

Place And Duration Of Study: Department of Operative, DIEKIOHS (DUHS) and NED University, Karachi, from February to June 2011.

Effect of different irradiation times on microhardness and depth of cure of a nanocomposite resin.

Objective: To determine the microhardness and depth of cure of nanocomposite using different irradiation times on both upper and lower surfaces of composite material.

Study Design: In-vitro experimental study design.

Place And Duration Of Study: Dr.