Partial epithelial-mesenchymal transition during enamel development.

Objectives: We set out to investigate whether a hybrid stem-like p-EMT phenotype develops during murine molar enamel development in vivo.

Setting And Sample Population: Histology specimens incorporating molar tooth buds harvested from mice at post-natal day 4 (P4) were included in our studies.

Materials And Methods: We employed double immunofluorescence staining to analyze the simultaneous expression of the epithelial marker E-cadherin and the mesenchymal marker N-cadherin in histology specimens with tooth buds harvested from P4 mice.

Reprogramming oral epithelial keratinocytes into a pluripotent phenotype for tissue regeneration.

Objectives: We set out to reprogram adult somatic oral epithelial keratinocytes into pluripotent cells for regenerative dentistry.

Setting And Sample Population: Immortalized murine oral keratinocyte cell (IMOK) line raised from adult mouse mucosa were cultured in vitro in our studies.

Materials And Methods: Adult murine oral epithelial keratinocytes were chronically treated with TGF-β1 in vitro, and the expression of Oct4, Nanog, Sox2 and Nestin, as well as specific homeobox Gata and Pax gene family members were investigated.

Serotonin contributes to the in vitro production of a biomimetic enamel-like material from reprogrammed oral epithelial keratinocytes.

Objectives: To evaluate the role of serotonin in the development of a biomimetic enamel-like material in vitro.

Setting And Sample Population: Immortalized murine oral keratinocytes raised from adult mouse mucosa were cultured in vitro. In addition, specimens incorporating molar tooth buds harvested from mice were included in our studies.