Validated spectrophotometric and spectrofluorimetric methods for determination of dapoxetine hydrochloride and dosulepin hydrochloride in their dosage forms using mercurochrome.

Validated, simple, rapid and sensitive spectrophotometric and spectrofluorimetric methods were developed for the determination of dapoxetine HCl and dosulepin HCl. The spectrophotometric method (I) was based on a binary complex formation between each drug and mercurochrome (MER) in acetate buffer (pH 3.5) with maximum absorbance at 557 nm.