Publications by authors named "Fawei Liao"
Unraveling radiation-induced skeletal muscle damage: Insights from a 3D human skeletal muscle organoid model.
Biochim Biophys Acta Mol Cell Res
October 2024
Background: Three-dimensional (3D) organoids derived from human pluripotent stem cells (hPSCs) have revolutionized in vitro tissue modeling, offering a unique opportunity to replicate physiological tissue organization and functionality. This study investigates the impact of radiation on skeletal muscle response using an innovative in vitro human 3D skeletal muscle organoids (hSMOs) model derived from hPSCs.
Methods: The hSMOs model was established through a differentiation protocol faithfully recapitulating embryonic myogenesis and maturation via paraxial mesodermal differentiation of hPSCs.
Article Synopsis
- Skeletal muscle ischemia-reperfusion (IR) injury presents serious challenges that existing 2D cell cultures and animal models often fail to replicate, limiting their effectiveness for human applications.
- The study introduces a novel 3D in vitro model using human skeletal muscle spheroids derived from human pluripotent stem cells (hPSCs) to better mimic human muscle development and the mechanisms behind IR injury.
- By establishing these muscle spheroids and co-culturing them with spinal cord spheroids, the research enhances our understanding of muscle development and provides a platform for studying IR injury, which could lead to advances in drug testing and personalized medicine.
Contusion concomitant with ischemia injury to skeletal muscles is common in civilian and battlefield trauma. Despite their clinical importance, few experimental studies on these injuries are reported. The present study established a rat skeletal muscle contusion concomitant with ischemia injury model to identify skeletal muscle alterations compared with contusion injury or ischemia injury.
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