Chromatographic analysis and pK evaluation of active pharmaceutical ingredients in anti-metastatic breast cancer: Green vs. conventional RPLC.

This study aimed to determine the chromatographic retention and dissociation/protonation constant (pK) values of lapatinib and tamoxifen, key drugs used in metastatic breast cancer treatment, at 37°C using both conventional and green high-performance liquid chromatography (HPLC) methods. Qualitative analysis was conducted on an XTerra C18 column (250 ×4.6 mm I.