Method in CRISPR/Cas System: An Expedite and Powerful Booster.

The CRISPR/Cas system has stood in the center of attention in the last few years as a revolutionary gene editing tool with a wide application to investigate gene functions. However, the labor-intensive workflow requires a sophisticated pre-experimental and post-experimental analysis, thus becoming one of the hindrances for the further popularization of practical applications. Recently, the increasing emergence and advancement of the methods play a formidable role to support and boost experimental work.

Network analysis of targets showing the potential oncogenic role of in colorectal cancer.

Background: is a member of the Kruppel-like factor, subfamily of zinc finger proteins that are involved in cancers. functions as a transcription factor and regulates the diverse protein-coding genes (PCGs) in colorectal cancer (CRC). However, the long non-coding RNAs (lncRNAs) regulated by in CRC are currently unknown.

Function and regulation annotation of up-regulated long non-coding RNA LINC01234 in gastric cancer.

Background: Accumulated evidences indicate that long non-coding RNAs (lncRNAs) participate in many biological mechanisms. Moreover, it acts as an essential regulator in various human diseases such as gastric cancer (GC). Nevertheless, the comprehensive regulatory roles and clinical significance of most lncRNAs in GC are not fully understood.