Optical detection based spot test on electrospun nanofibers for glioblastoma cells.

Paper-based diagnosis is enabled to be used in many applications in global health due to its low cost and simplicity of operation. Electrospun nanofibers (ESNFs) are useful platforms to prepare paper-based diagnostics. Herein, bead-free ESNFs were formed by electrospinning using polystyrene (PS) and poly (ethylene glycol) (PEG) polymers as a paper-based substrate.

A colorimetric immunoassay for the detection of human vascular endothelial growth factor 165 (VEGF) based on anti-VEGF-iron oxide nanoparticle conjugation.

Vascular endothelial growth factor (VEGF) is an indispensable element in many physiological processes, while alterations in its level in the circulating system are signs of pathology-associated diseases. Therefore, its precise and selective detection is critical for clinical applications to monitor the progression of the pathology. In this study, an optical immunoassay biosensor was developed as a model study for detecting recombinant VEGF.

Development of an Enzymatic Biosensor Using Glutamate Oxidase on Organic-Inorganic-Structured, Electrospun Nanofiber-Modified Electrodes for Monosodium Glutamate Detection.

Herein, dendrimer-modified montmorillonite (Mt)-decorated poly-Ɛ-caprolactone (PCL) and chitosan (CHIT)-based nanofibers were prepared. Mt was modified with a poly(amidoamine) generation 1 (PAMAM) dendrimer, and the obtained PAMAM-Mt was incorporated into the PCL-CHIT nanofiber's structure. The PCL-CHIT/PAMAM-Mt nanofibers were conjugated with glutamate oxidase (GluO) to design a bio-based detection system for monosodium glutamate (MSG).

Copolymer based multifunctional conducting polymer film for fluorescence sensing of glucose.

A simple, rapid and effective fluorescence sensing platform has been fabricated using a fluorescent conducting polymer surface. For this purpose, a rhodamine based electroactive monomer (RDC) and a functional group containing monomer (SNS) have been copolymerized to develop a conducting polymer based sensor platform having a fluorescence and enzyme-binding surface on ITO electrode. The proposed fluorescence sensing mechanism for detection of glucose is related to the consumption of dissolved oxygen at the double layer of the electrode which is fluorescence quenching agent by glucose-GOx reaction.

Biofunctionalization of PAMAM-montmorillonite decorated poly (Ɛ-caprolactone)-chitosan electrospun nanofibers for cell adhesion and electrochemical cytosensing.

The construction and biofunctionalization of the poly (Ɛ-caprolactone) (PCL)-chitosan (CHIT) nanofibrous mats, which included Polyamidoamine (PAMAM) dendrimer modified montmorillonite (Mt), for the cell adhesion and electrochemical cytosensing were accomplished in this report. After the intercalation of the PAMAM generation zero dendrimer into the Mt, PAMAM-Mt decorated PCL-CHIT electrospun nanofibers were formed. The addition of PAMAM caused the decrease of contact angle of PCL-CHIT nanofibers.

Rhodamine functionalized conducting polymers for dual intention: electrochemical sensing and fluorescence imaging of cells.

We report here the electrochemical co-polymerization of two functional monomers, one containing fluorescent rhodamine dye (RF) and the other monomer having amine groups (RD), onto electroactive Indium Tin Oxide (ITO) glass. After one step preparation of these surfaces, a three peptide called ArginylGlysylAspartic acid (RGD) was immobilized via EDC chemistry by using amine groups (P(RF-co-RD)/RGD) of the co-polymer, for further use in various bio-applications such as cell adhesion and imaging as well as electrochemical cell sensing. The resultant RGD bound and also fluorescent platforms were utilized as targeted adhesion materials towards integrin avb3 receptor positive (U87-MG) cells and the selectivity was checked by using HaCaT cells as a control.

Testing of bioactive-nanovesicles on hepatotoxicity of atypical antipsychotics via digital holography.

Atypical antipsychotic drugs induce hepatic toxicity. Thus, it is of importance to eliminate the side effects of these drugs. Herein we describe the preparation of nanoemulsions with a dietary supplement; wheat germ oil (WGO), to ameliorate the liver damage induced by clozapine and olanzapine.