Acinetobacter lwoffi peritonitis in peritoneal dialysis: two cases report.

Peritonitis is an important cause of morbidity and technique failure in peritoneal dialysis. Herein, we report peritonitis related to Acinetobacter lwoffi in two patients on peritoneal dialysis. The first case is a 63-year-old patient treated by automated peritoneal dialysis admitted with abdominal pain.

Unusual Cause of Peritonitis in Peritoneal Dialysis Patient Related to Streptococcus salivarius.

Peritonitis remains the most common serious complication associated with peritoneal dialysis (PD). The Gram-positive peritonitis with Staphylococcus aureus and coagulase-negative Staphylococcus are the most frequent causes, whereas Gram-positive and Gram-negative species remain less frequent. We report an uncommon case of PD-related peritonitis due to Streptococcus salivarius, known as nonpathogen Gram-positive bacteria of oral flora.

A rapid and easy method for the DNA extraction from Cryptococcus neoformans.

DNA isolation from C. neoformans is difficult due to a thick and resistant capsule. We have optimized a new and rapid DNA isolation method for Cryptococcus using a short urea treatment followed by a rapid method using a chelex resin suspension.

Two new media Pinus halepensis seed agar and blackberry agar for rapid identification of Cryptococcus neoformans.

Cryptococcus neoformans is an encapsulated yeast-like fungus that causes life-threatening infections, particularly in immunocompromised patients. The formation of brown pigment on many media described in the literature, such as that in Niger seed (Guizotia abyssinica) agar, has been used to identify C. neoformans.