Recent advances in nanoformulation-based delivery for cancer immunotherapy.

Cancer is one of the leading causes of mortality worldwide, and its treatment faces several challenges. Phytoconstituents derived from recently discovered medicinal plants through nanotechnology potentially target cancer cells via PI3K/Akt/mTOR pathways and exert their effects selectively through the generation of reactive oxygen species through β-catenin inhibition, DNA damage, and increasing caspase 3/9 and p53 expression. These nanocarriers act specifically against different cancer cell lines such as HT-29, MOLT-4 human leukemia cancer and MCF-7 cell lines SKOV-3, Caov-3, SW-626, HepG2, A-549, HeLa, and MCF-7.

Biochemical characterization of bioinspired nanosuspensions from Swertia chirayita extract and their therapeutic effects through nanotechnology approach.

Swertia chirayita is used as a traditional medicinal plant due to its pharmacological activities, including antioxidant, antidiabetic, antimicrobial, and cytotoxic. This study was aimed to evaluate the therapeutic efficacy of newly synthesized nanosuspensions from Swertia chirayita through nanotechnology for enhanced bioactivities. Biochemical characterization was carried out through spectroscopic analyses of HPLC and FTIR.

An Insight into Biochemical Characterization and Explorations of Antioxidant, Antibacterial, Cytotoxic, and Antidiabetic Activities by Nanosuspensions.

Background: is a frequently utilized traditional medicinal plant renowned for its pharmacological attributes, particularly in the realm of treating infectious diseases. This current study aims to comprehensively assess the properties of freshly prepared nanosuspensions derived from extracts, with a focus on their cost-effective potential in various areas, including antioxidant, antibacterial, cytotoxic, and antidiabetic activities.

Methods: Biochemical characterization of nanosuspensions by high-performance liquid chromatography (HPLC) and Fourier-transform infrared spectroscopy (FTIR) analyses.

The role of mitochondria and mitophagy in cell senescence.

Mitochondrial malfunction and cell senescence have been defined as the hallmarks of aging. Cell senescence leads to the loss of health allied with aging. While deciphering the complex association between mitochondria and cellular senescence, it is observed that senescence has a two-faced nature being beneficial and hazardous.

Biochemical, structural characterization and evaluation of antioxidant, antibacterial, cytotoxic, and antidiabetic activities of nanosuspensions of bark extract.

is a traditional medicinal plant known for its anti-inflammatory, antidiabetic, antimicrobial, anticancer, and antioxidant properties. Its therapeutic efficacy using nanosuspensions is still unclear for treating infectious diseases. This study was designed to evaluate the bioactivities, biochemical characterization, and bioavailability of freshly prepared nanosuspensions of .

Nanotechnology approach for exploring the enhanced bioactivities, biochemical characterisation and phytochemistry of freshly prepared Mentha arvensis L. nanosuspensions.

Introduction: Mentha arvensis L. is the most valuable medicinal plant that possesses anti-inflammatory, hepatoprotective, antimicrobial, and antioxidant properties. There are few studies available in the literature about M.

and study of inhibitory potentials of α-glucosidase and acetylcholinesterase and biochemical profiling of in alloxan-induced diabetic rat models.

Objectives: Diabetes mellitus is a multifactorial chronic disease that affects the human population and it is the third most common cause of death worldwide. is used as popular folk medicine and its action against diabetes mellitus remains unclear. We investigated the inhibitory potentials of α-glucosidase, acetylcholinesterase, and biochemical profiling of in alloxan-induced diabetic rat models.

Nanotechnology Approach for Exploring the Enhanced Bioactivities and Biochemical Characterization of Freshly Prepared L. Nanosuspensions and Their Phytochemical Profile.

is one of the most commonly used medicinal plants as it exhibits several pharmacological activities such as antioxidant, antibacterial, anticancer, antidiabetic, and hemolytic. The purpose of this study was to apply the nanotechnology approach for exploring the enhanced bioactivities of freshly prepared L. nanosuspensions and the phytochemical profile of seed ethanolic extract.

Ultrasound Assisted Extraction and Characterization of Bioactives From V Roots to Evaluate Their Antioxidant and Medicinal Potential.

Worldwide, is used for the treatment of various ailments owing to the presence of bioactives of therapeutic potential. Current study was planned to extract bioactives from roots using methanol and water as solvents under stimulated effect of ultrasonic waves and characterize them to evaluate their potential benefits. Proximate analysis explored the presence of significant contents of protein, fats, fiber, organic and inorganic minerals.

Exopolysaccharides production from marine Bacillus strains and their antioxidant and bio-flocculant capacities.

Microbial exopolysaccharides (EPS) have gained high scientific concern due to their exceptional physicochemical features and high industrial applicability. Owing to their biotechnological importance, the present study was designed to screen and isolate the EPS-producing Bacillus strains based on their growth potential on specific media and colony morphologies. The bacterial isolates Bacillus subtilis Bs1-01, Bacillus licheniformis Bl1-02, and Bacillus brevis Bb1-04 showed excellent EPS production due to their shortened lag phase and abundant biomass production.

Association analysis of vitamin D receptor gene polymorphisms in North England population with Type 2 diabetes mellitus.

Background: Numerous diabetes susceptibility loci, include a region consisting vitamin D receptor gene found in chromosome 12q, have been known using genome wide screens.

Aim: The aim of present study is to probe the relationship between polymorphism of vitamin D receptor gene (single nucleotide polymorphisms) and type 2 diabetes mellitus (T2DM). Five hundred T2DM patients and 200 healthy subjects with normal HbA1c (≤ 5.

Aging - Oxidative stress, antioxidants and computational modeling.

Aging is a degenerative, biological, time-dependent, universally conserved process thus designed as one of the highest known risk factors for morbidity and mortality. Every individual has its own aging mechanisms as both environmental conditions (75%) and genetics (25%) account for aging. Several theories have been proposed until now but not even a single theory solves this mystery.

Vitamin D receptor FokI polymorphism in a Pakistani population with type 2 diabetes mellitus.

Present research was undertaken with the aim to assess the association of VDR gene FokI polymorphism with T2DM in local population. The study comprised of 100 T2DM patients (DG) and 50 normal individuals (CG) groups. Demographic parameters; age, gender, BMI and blood pressure were recorded.

Association of Vitamin D receptor gene I polymorphism with type 2 diabetes mellitus in Pakistani population.

Background: Type 2 diabetes mellitus (T2DM) is a complex metabolic disorder with strong genetic components. The reported association of vitamin D receptor (VDR) gene polymorphisms varies among ethnic groups.

Objectives: The present study was conducted to determine association of vitamin D receptor gene I (rs1544410 A>G) polymorphism with type 2 diabetes mellitus in Pakistani population.

Association of RAGE gene polymorphism with Type-2 diabetes mellitus in local population.

Objectives: Type-2 diabetes mellitus (T2DM) is an endocrine disease having a significant genetic component. Polymorphisms of many genes may affect hereditary vulnerability of the disease that is characterized by insulin resistance and islet disorder. As the genetic basis of T2DM can vary between ethnic groups, it is important to investigate the genetic link of T2DM in Pakistani populace.

Assessing universality of DNA barcoding in geographically isolated selected desert medicinal species of Fabaceae and Poaceae.

In pursuit of developing fast and accurate species-level molecular identification methods, we tested six DNA barcodes, namely ITS2, KLa, ITS2+K, ITS2+La, K+La and ITS2+K+La, for their capacity to identify frequently consumed but geographically isolated medicinal species of Fabaceae and Poaceae indigenous to the desert of Cholistan. Data were analysed by BLASTn sequence similarity, pairwise sequence divergence in TAXONDNA, and phylogenetic (neighbour-joining and maximum-likelihood trees) methods. Comparison of six barcode regions showed that ITS2 has the highest number of variable sites (209/360) for tested Fabaceae and (106/365) Poaceae species, the highest species-level identification (40%) in BLASTn procedure, distinct DNA barcoding gap, 100% correct species identification in BM and BCM functions of TAXONDNA, and clear cladding pattern with high nodal support in phylogenetic trees in both families.

Epidemiology of hepatitis C viral infection in Faisalabad, Pakistan: a retrospective study (2010-2012).

Background: Hepatitis viral infections are major health challenge leading to high morbidity and mortality worldwide.

Objectives: Although the magnitude of hepatitis in Pakistan has been well documented, information regarding the prevalence of hepatitis C virus (HCV) infection in Faisalabad, Pakistan is scarce. The present retrospective study was undertaken to determine the epidemiology of HCV in Faisalabad, Pakistan.

Prevalence and risk factors of HIV in Faisalabad, Pakistan -A retrospective study.

Background & Objective: Although the magnitude of HIV in Pakistan has been well documented, but no record of HIV prevalence in Faisalabad region exists. A retrospective study was carried out at Sexually Transmitted Infections (STIs) clinic, District Headquarter (DHQ) hospital, Faisalabad, Pakistan to find out the prevalence of HIV and related risk factors.

Methods: Between March, 2010 and December, 2012, a total of 31040 subjects were either interviewed or their medical records were reviewed.

Sexually transmitted infections in Pakistan.

Background And Objectives: Sexually transmitted infections (STIs) represent a major global health problem leading to morbidity, mortality and stigma. Prior to this study there was no information on the prevalence and knowledge of STIs in Faisalabad, Pakistan.

Design And Setting: Prospective, cross-sectional study in patients attending STI clinics from July 2006 to September 2009.