Mismatch Amplification Mutation Assay-Based Real-Time PCR for Rapid Detection of Neisseria gonorrhoeae and Antimicrobial Resistance Determinants in Clinical Specimens.

Molecular methods are often used for detection, but complete definition of antimicrobial resistance (AMR) patterns still requires phenotypic tests. We developed an assay that both identifies and detects AMR determinants in clinical specimens. We designed a mismatch amplification mutation assay (MAMA)-based SYBR green real-time PCR targeting one -specific region (); mosaic alleles (Asp345 deletion [Asp345del], Gly545Ser) associated with decreased susceptibility to cephalosporins; and alterations conferring resistance to ciprofloxacin (GyrA Ser91Phe), azithromycin (23S rRNA A2059G and C2611T), and spectinomycin (16S rRNA C1192T).

Stability of hematological analytes depends on the hematology analyser used: a stability study with Bayer Advia 120, Beckman Coulter LH 750 and Sysmex XE 2100.

Aim: With the availability of newer hematology analysers, novel measurement techniques are being introduced into daily routine. As analyte stability may differ according to the employed measurement technique, the aim of this study was to assess the stability of hematologic analytes on different hematology analysers.

Methods: We investigated the effect of storage time and storage temperature on sample stability on three newer analytical systems (Advia 120, Bayer Diagnostics; XE 2100, Sysmex and LH 750, Beckman Coulter).