A comparative study of 589 and 532 nm low level laser irradiation effects on normal human peripheral blood mononuclear cells viability.

Objective: To assess how laser light affects blood lymphocyte viability in vitro.

Methods: The comparative study was conducted from November 2021 to April 2022 at the Department of Medical Physics, Mustansiriyah University, Baghdad, Iraq, and comprised blood samples collected from healthy adults with no medical history of major illnesses or history of taking medications for major diseases.Low-level laser 589nm and 532nm was used at 30 J/cm², 50 J/cm² and 70J/cm² energy intensities and three different post-exposure time; immediately, 1h and 2h after radiation exposure.