Microstructure and color stability of calcium silicate-based dental materials exposed to blood or platelet-rich fibrin.

Objectives: To investigate the effects of blood and platelet-rich fibrin (PRF), commonly used scaffolds in regenerative endodontic treatment (RET), on the hydration, microstructure, and color stability of three hydraulic calcium silicate cements (HCSCs), OrthoMTA, RetroMTA, and TotalFill-BC-RRM.

Materials And Methods: The HCSCs were prepared and placed into polyethylene molds and transferred to Eppendorf tubes containing PRF, blood, or PBS and then incubated for 1 week or 1 month. The microstructure and hydration of the cements were studied by scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD).

An Electroconductive, Thermosensitive, and Injectable Chitosan/Pluronic/Gold-Decorated Cellulose Nanofiber Hydrogel as an Efficient Carrier for Regeneration of Cardiac Tissue.

Myocardial infarction is a major cause of death worldwide and remains a social and healthcare burden. Injectable hydrogels with the ability to locally deliver drugs or cells to the damaged area can revolutionize the treatment of heart diseases. Herein, we formulate a thermo-responsive and injectable hydrogel based on conjugated chitosan/poloxamers for cardiac repair.

Multilayered Mesoporous Composite Nanostructures for Highly Sensitive Label-Free Quantification of Cardiac Troponin-I.

Cardiac troponin-I (cTnI) is a well-known biomarker for the diagnosis and control of acute myocardial infarction in clinical practice. To improve the accuracy and reliability of cTnI electrochemical immunosensors, we propose a multilayer nanostructure consisting of FeO-COOH labeled anti-cTnI monoclonal antibody (FeO-COOH-Ab) and anti-cTnI polyclonal antibody (Ab) conjugated on Au-Ag nanoparticles (NPs) decorated on a metal-organic framework (Au-Ag@ZIF-67-Ab). In this design, FeO-COOH was used for separation of cTnI in specimens and signal amplification, hierarchical porous ZIF-67 extremely enhanced the specific surface area, and Au-Ag NPs synergically promoted the conductivity and sensitivity.

Thermosensitive alginate-gelatin-nitrogen-doped carbon dots scaffolds as potential injectable hydrogels for cartilage tissue engineering applications.

Hybrid injectable and biodegradable hydrogels based on oxidized alginate/gelatin and containing nitrogen-doped carbon dots (NCDs) as a reinforcement have been fabricated and crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/-hydroxysuccinimide (NHS) as the chemical crosslinking agents in the hydrogel system. The idea of composite hydrogels relies on the assumption that they supply a microenvironment that is convenient for the exchange of nutrients a porous structure and cell proliferation and have mechanical characteristics that approximately match natural tissue. The effect of the NCD content on the morphology structure, mechanical strength, swelling ratio, and biodegradation has been investigated.

study of alginate-gelatin scaffolds incorporated with silica NPs as injectable, biodegradable hydrogels.

Porous substrates composed of biodegradable polymers and nanoparticles have found extensive use as three-dimensional (3D) scaffolds to regenerate damaged tissues through the incorporation of cells or growth factors. Here, injectable thermally responsive hydrogels based on SiO nanoparticles (NPs), alginate, and gelatin biopolymers, with possible utilization for cartilage tissue engineering, are introduced. The nanocomposites contain different amounts of SiO NPs for reinforcement and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/-hydroxysuccinimide (NHS) for chemical crosslinking of polymer chains in the 3D hydrogel network.

Injectable hydrogels based on oxidized alginate-gelatin reinforced by carbon nitride quantum dots for tissue engineering.

Stem cell treatment is promising in the various disorders treatment, but its effect is confined by the adverse conditions in the damaged tissues. The utilization of hydrogels has been suggested as a procedure to defeat this issue by developing the engraftment and survival of injected stem cells. Specifically, injectable hydrogels have drawn much attention due to their shape adaptability, ease of use, and the capability to reach body parts that are hard to access.

Extraction of Hydroxyapatite Nanostructures from Marine Wastes for the Fabrication of Biopolymer-Based Porous Scaffolds.

Three-dimensional porous nanocomposites consisting of gelatin-carboxymethylcellulose (CMC) cross-linked by carboxylic acids biopolymers and monophasic hydroxyapatite (HA) nanostructures were fabricated by lyophilization, for soft-bone-tissue engineering. The bioactive ceramic nanostructures were prepared by a novel wet-chemical and low-temperature procedure from marine wastes containing calcium carbonates. The effect of surface-active molecules, including sodium dodecyl sulfate (SDS) and hexadecyltrimethylammonium bromide (CTAB), on the morphology of HA nanostructures is shown.

Missense Mutation in Fam83H Gene in Iranian Patients with Amelogenesis Imperfecta.

Background: Amelogenesis Imperfecta (AI) is a disorder of tooth development where there is an abnormal formation of enamel or the external layer of teeth. The aim of this study was to screen mutations in the four most important candidate genes, ENAM, KLK4, MMP20 and FAM83H responsible for amelogenesis imperfect.

Methods: Geneomic DNA was isolated from five Iranian families with 22 members affected with enamel malformations.

Hydroxyapatite nanocrystals: simple preparation, characterization and formation mechanism.

Crystalline hydroxyapatite (HAP) nanoparticles and nanorods have been successfully synthesized via a simple precipitation method. To control the shape and particle size of HAP nanocrystals, coordination ligands derived from 2-hydroxy-1-naphthaldehyde were first prepared, characterized by Fourier transform infrared (FT-IR) and proton nuclear magnetic resonance ((1)H-NMR) spectroscopies, and finally applied in the synthesis process of HAP. On the other hand, the HAP nanocrystals were also characterized by several techniques including powder X-ray diffraction (XRD), field-emission scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM).

Particle size and shape modification of hydroxyapatite nanostructures synthesized via a complexing agent-assisted route.

In this work, hydroxyapatite (HAP), Ca10(PO4)6(OH)2, nanostructures including nanorods, nanobundles and nanoparticles have been prepared via a simple precipitation method. In the present method, Ca(NO3)2·4H2O and (NH4)2HPO4 were used as calcium and phosphorus precursors, respectively. Besides, the Schiff bases derived from 2-hydroxyacetophenone and different diamines were used as complexing agents for the in situ formation of Ca(2+) complexes.

Sonochemical preparation of pure t-LaVO4 nanoparticles with the aid of tris(acetylacetonato)lanthanum hydrate as a novel precursor.

Herein a simple and fast method is introduced for the synthesis of lanthanum orthovanadate (LaVO4) nanoparticles under ultrasound irradiation. The effect of tris(acetylacetonato)lanthanum hydrate ([La(acac)3·3H2O]) and La(OAc)3 as two different precursors on the morphology and phase purity of LaVO4 was investigated. To optimum the particle size of the products, sonication time and the kind of surfactants have been changed.

Sonochemical synthesis and characterization of lead iodide hydroxide micro/nanostructures.

For the first time, micro/nano-sized lead iodide hydroxide; Pb(OH)I, has been successfully prepared via a simple ultrasonic method. In this method, lead nitrate and lithium iodide were applied as starting reagents to fabricate Pb(OH)I micro/nanostructures at different conditions. The effect of different surfactants like N,N-bis(salicylidene)-ethylenediamine (H2salen), sodium dodecyl sulfate (SDS) and polyvinylpyrrolidone (PVP), sonication time, and ultrasonic intensity on the morphology and particle size of the products has been investigated.

Sonochemical synthesis of silver vanadium oxide micro/nanorods: solvent and surfactant effects.

In this investigation, a facile sonochemical route has been developed for the preparation of silver vanadium oxide (SVO) micro/nanorods by using silver salicylate and ammonium metavanadate as silver and vanadate precursor, respectively. Here, silver salicylate, [Ag(HSal)], is introduced as a new silver precursor to fabricate AgVO(3) nanorods. The effect of numerous solvents and surfactants on the morphology and sonochemical formation mechanism of AgVO(3) nanorods was studied.